文殊兰叶子化学成分及抗炎活性研究  

Chemical Constituents from Leaves of Crinum asiaticum var. sinicum and Their Anti-inflammatory Activities

作　　者：王昕 范青飞[1] 周兰 毛鹏伟 张玉梅[1] 宋启示[1] WANG Xin;FAN Qing-fei;ZHOU Lan;MAO Peng-wei;ZHANG Yu-mei;SONG Qi-shi(Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming 650223;University of Chinese Academy of Sciences,Beijing 100049,China;Puer University,Puer 665000,China)

机构地区：[1]中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室,昆明650223 [2]中国科学院大学,北京100049 [3]普洱学院,普洱665000

出　　处：《天然产物研究与开发》2018年第8期1354-1360,共7页Natural Product Research and Development

基　　金：中国科学院战略性先导科技专项A(XDA12050302);中国科学院"一三五"专项(XTBG-F02)

摘　　要：本文对西双版纳傣药痹通剂的配方植物文殊兰的叶子进行了化学成分分离以及抗炎活性研究。通过色谱柱分离纯化,从文殊兰叶子的甲醇提取物中分离得到13个化合物,并运用现代波谱学技术分别鉴定为:7,4'-二羟基黄烷(1)、7,4'-二羟基-8-甲基黄烷(2)、杜鹃素(3)、stigmast-4-ene-3β,6β-diol(4)、byzantionoside B(5)、daucosterol+stigmasterol-3-O-glucopyranoside(6)、(6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside(7)、5-hydroxy-6,7-methylenedioxy-2-methylchromone(8)、正丁基吡喃果糖苷(9)、豆甾醇(10)、豆甾醇苷(11)、β-谷甾醇(12)、胡萝卜苷(13),其中化合物2~8为首次从该植物中分离得到。文殊兰叶子提取物具有显著的NO抑制活性,即显著的抗炎活性,本次实验分离得到的化合物7,4'-二羟基-8-甲基黄烷(2)和杜鹃素(3)具有一定的抗炎活性。This paper reported the chemical constituents and anti-inflammatory activities of leaves of Crinum asiaticum var.sinicum,an herb for treatment of rheumatoid arthritis in Xishuangbanna Dai medicine.Thirteen compounds were isolated and purified from C.asiaticum var.sinicum by using column chromatography and identified by chemical and spectral analyses.Their structures were elucidated as 7,4′-dihydroxyflavan(1),7,4′-dihydroxy-8-methyl-flavan(2),farrerol(3),stigmast-4-ene-3β,6β-diol(4),byzantionoside B(5),daucosterol+stigmasterol-3-O-glucopyranoside(6),(6 R,9 R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside(7),5-hydroxy-6,7-methylenedioxy-2-methylchromone(8),n-butyl-β-D-fructopyranoside(9),stigmasterol(10),stigmasterol-3-O-β-D-fructopyranoside(11),β-sitosterol(12),daucosterol(13),among which compounds 2-8 were obtained from this plant for the first time.Extracts from leaves of C.asiaticum var.sinicum showed significant NO inhibitory activities,i.e.anti-inflammatory activities,as well as compounds 7,4′-dihydroxy-8-methyl-flavan(2),farrerol(3),which enriched the study of C.asiaticum var.sinicum and will be conducive to the exploiting of its medicinal value.

关 键 词：文殊兰 化学成分 结构鉴定 抗炎活性 

分 类 号：R932[医药卫生—生药学] Q94[医药卫生—药学]