单羽火筒树化学成分研究  

Chemical Constituents from Leea asiatica

作　　者：杨珺[1,2] 黄铁坚[3] 罗吉凤 李恒[1] 王跃虎[1,2] YANG Jun;HUANG Tie-jian;LUO Ji-feng;LI Heng;WANG Yue-hu(Key Laboratory of Economic Plants and Biotechnology,and Yunnan Key Laboratory for Wild Plant Resource, Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;Southeast Asia Biodiversity Research Institute,Chinese Academy of Sciences,Yezin,Nay Pyi Taw 05282,Myanmar;Bo Hong Industrial Company Limited,Wandin,Ruili 678500,China)

机构地区：[1]中国科学院昆明植物研究所资源植物与生物技术重点实验室及云南省野生资源植物研发重点实验室,昆明650201 [2]中国科学院东南亚生物多样性研究中心,缅甸奈比多耶津05282 [3]瑞丽市畹町经济开发区博鸿实业有限公司,畹町678500

出　　处：《天然产物研究与开发》2018年第8期1382-1386,共5页Natural Product Research and Development

基　　金：中国科学院东南亚生物多样性研究中心(Y4ZK111B01)

摘　　要：采用色谱柱层析及半制备高效液相色谱的分离方法,以及质谱和核磁共振波谱的鉴定方法,从云南省德宏市民间利用的抗肿瘤药用植物单羽火筒树(九节狸)全株中分离、鉴定出了11个化合物,即山柰酚(1)、山萘酚-3-O-α-L-吡喃鼠李糖苷(2)、槲皮素(3)、异槲皮苷(4)、槲皮素-3-O-α-L-吡喃鼠李糖苷(5)、杨梅素(6)、杨梅素-3-O-α-L-吡喃鼠李糖苷(7)、Europetin(8)、Europetin 3-O-α-L-rhamnopyranoside(9)、(-)-儿茶素(10)和山楂酸(11)。采用MTS法测试了乙酸乙酯部位及11个化合物对5种肿瘤细胞株的体外细胞毒活性,结果表明单羽火筒树乙酸乙酯部位对人白血病HL-60细胞(IC_(50)=21.76μg/mL)、人肝癌SMMC-7721细胞(IC_(50)=17.04μg/mL)和人肺癌A-549细胞株(IC_(50)=18.00μg/mL)的生长有抑制活性,从乙酸乙酯部位分离出来的化合物1对肝癌细胞株(SMMC-7721)的生长有抑制活性(IC_(50)=27.61μg/mL)。Through column chromatography and semi-preparative high performance liquid chromatography(HPLC),11 compounds were isolated from the whole plants of Leea asiatica with the common name of Jiu-Jie-Li,a traditional antitumor ethnomedicine used by local people in Dehong of Yunnan Province.These compounds were identified as kaempferol(1),kaempferol-3-O-α-L-rhamnopyranoside(2),quercetin(3),isoquercitrin(4),quercitrin(5),myricetin(6),myricetin-3-O-α-L-rhamnopyranoside(7),europetin(8),europetin 3-O-α-L-rhamnopyranoside(9),ent-catechin(10),and maslinic acid(11)by MS and NMR spectra.The cytotoxic activity against 5 tumor cell lines of the EtOAc-soluble part and 11 compounds were evaluated by an MTS assay.The result showed that the EtOAc-soluble part exhibited growth inhibitory activity against human leukemia HL-60(IC 50=21.76μg/mL)cell line,human lung cancer A-549(IC 50=18.00μg/mL)cell line,and human liver cancer SMMC-7721(IC 50=17.04μg/mL)cell line,and kaempferol(1)from the the EtOAc-soluble part was active against SMMC-7721 cells with an IC 50 value of 27.61μg/mL.

关 键 词：火筒树科 单羽火筒树 黄酮 细胞毒 肿瘤 

分 类 号：R284.2[医药卫生—中药学]