水稻短根突变体Osksr6的遗传分析和基因定位  

Genetic Analysis and Gene Mapping of a Short Root Mutant Osksr6 in Rice(Oryza sativa L.)

作　　者：叶金 诸周洁 姚捷 朱俊兆 王晓飞[3] 丁沃娜[2] YE Jin;ZHU Zhoujie;YAO Jie;ZHU Junzhao;WANG Xiaofei;DING Wona(School of Marine Science,Ningbo University,Ningbo,Zhejiang 315211,China;College of Science and Technology,Ningbo University,Ningbo,Zhejiang 315211,China;School of Agriculture and Food Science,Zhejiang Agriculture and Forestry University,Hangzhou 311300,China)

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]宁波大学科学技术学院,浙江宁波315212 [3]浙江农林大学农业与食品科学学院,浙江杭州311300

出　　处：《西北植物学报》2018年第7期1222-1227,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31400263);宁波市自然科学基金(2017A610291)

摘　　要：该研究对从甲基磺酸乙酯(EMS)诱变的水稻突变体库中筛选到的一个短根突变体Osksr6(Oryza sativa kasalath short root 6)进行了表型鉴定、遗传分析与基因定位。结果表明:(1)生长7d的突变体Osksr6与野生型相比,株高与不定根数量差异不明显,但主根变短61.98%、不定根变短46.42%,侧根的发生与根毛的伸长也受不同程度抑制;成熟期的Osksr6分蘖数明显减少,总穗长与结实率均较野生型差。(2)遗传分析结果显示,突变体Osksr6的短根性状受1对隐性基因控制。(3)利用图位克隆技术,将突变基因OsKSR6定位于3号染色体InDel标记28420k和28880k之间,物理距离约460kb,该区间没有已报道的与根系发育相关的基因。该研究为进一步研究水稻根系生长的分子机理奠定了基础。A rice short-root mutant Osksr 6 was identified from an ethylmethane sulfonate(EMS)-mutagenized rice library.Phenotypic observation,genetic analysis and gene mapping analysis of Osksr 6 were carried out.Results showed that:(1)at the 7 d old stage,there was no significant difference in plant height and the number of adventitious roots between the wild type and Osksr6,while the length of primary and adventitious roots of Osksr 6 was decreased by 61.98%and 46.42%compared with the wild type,respectively.Furthermore,the development of lateral roots and root hairs in Osksr 6 was also inhibited to varying degrees.At the maturation stage,the tiller number of Osksr 6 significantly decreased,and the panicle length and seed rate of Osksr 6 were also reduced.(2)Genetic analysis showed that the mutant phenotype was controlled by a single pair of recessive nuclear gene.(3)Map-based cloning analysis located OsKSR 6 to a 460 kb region between newly designed InDel markers 28420k and 28880k on chromosome 3.There is no gene reported to be involved in root development in this region.This result will be helpful for the cloning of OsKSR 6 and further characterization of molecular genetic mechanisms underlying root architecture in rice.

关 键 词：水稻 短根突变体 遗传分析 基因定位 

分 类 号：Q343.13[生物学—遗传学] Q343.17