不同性别C57BL/6小鼠肠道胆汁酸组成的差异性研究  

Gender Difference in Intestinal Bile Acid Profiles in C57BL/6 Mice

作　　者：张聪聪 吴俊芳[1] 王玉兰[1] ZHANG Cong-cong;WU Jun-fang;WANG Yu-lan(State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan(Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences),Wuhan 430071,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]波谱与原子分子物理国家重点实验室,武汉磁共振中心(中国科学院武汉物理与数学研究所),湖北武汉430071 [2]中国科学院大学,北京100049

出　　处：《波谱学杂志》2018年第3期328-337,共10页Chinese Journal of Magnetic Resonance

基　　金：国家重点基础研究发展计划(“973”计划)资助项目(2012CB934004);湖北省自然科学基金资助项目(2017CFB536).

摘　　要：随着人们对宿主肠道菌群的日益关注,关于胆汁酸与肠道菌群之间复杂关系的研究也越来越多.因肠道组织结构的复杂性和肠道菌群分布差异性,不同肠段胆汁酸的分布、表达及其生物学功能仍有待进一步研究.本文采用基于超高效液相色谱-三重四极杆-质谱联用(UPLC-QqQ-MS)的靶向代谢组分析结合基于核磁共振(NMR)波谱的非靶向代谢组分析方法,通过比较不同性别C57BL/6小鼠肠道各区段胆汁酸及粪样代谢组的变化水平,发现雌、雄小鼠不同肠段中胆汁酸的分布各不相同.另外,肠道下段各胆汁酸的性别差异性更为明显,以盲肠的性别差异性最为显著,这可能与盲肠的菌群微生态更为丰富有关.该研究为不同性别模型小鼠胆汁酸的代谢表型及其与肠道菌群的相互作用提供了基础研究数据.Gut microbiota is important for human health.The complex relationship between intestinal flora and bile acid profile has been studied previously.However,few previous studies have determined the profile and functions of bile acids in different intestinal segments,and whether they are related to the distribution of intestinal flora.In this paper,we measured the distribution of bile acids in different intestinal segments of male and female C57BL/6 mice with ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry(UPLC-QqQ-MS)based targeted metabonomics analysis and nuclear magnetic resonance(NMR)based untargeted metabonomics analysis.Gender difference in distributions of bile acids were observed,which was more evident in the lower parts of intestine than in the upper part of intestine,especially in the cecum segment.This might be related to the abundance of gut microflora in different intestine segments.This study provided metabolic phenotypes of bile acids in mice of different genders,and demonstrated their interactions with gut microbiota.

关 键 词：代谢组学 胆汁酸 C57BL/6小鼠 性别差异性 肠道菌群 

分 类 号：O482.53[理学—固体物理]