百部汤对博来霉素诱导小鼠肺纤维化的抑制作用  

Protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice

作　　者：谢伟娜 丁奇[1] 孙静 张朝凤[1] 张勉[1] 许翔鸿[1] XIE Weina;DING Qi;SUN Jing;ZHANG Chaofeng;ZHANG Mian;XU Xianghong(College of Traditional Chinese Medicine,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学中药学院,南京211198

出　　处：《中国药科大学学报》2018年第4期483-489,共7页Journal of China Pharmaceutical University

摘　　要：研究百部汤对博来霉素诱导的肺纤维化小鼠的抑制作用。一次性气管滴注博来霉素建立肺纤维化小鼠模型,每天灌胃给予百部汤1次,连续给药14 d,以尼达尼布作为阳性对照。分离肺组织,计算肺系数,测定羟脯胺酸含量,HE和Masson染色观察肺组织病理改变情况。结果显示,模型组小鼠体重下降,肺系数、羟脯氨酸含量、肺组织炎性、胶原等指标均明显升高;百部汤醇提物和水提物均有改善各项病理指标的作用或趋势,表明二者均有改善小鼠肺纤维化的作用,但醇提物效果优于水提物;而由不同化学型(分别主含新对叶百部碱、对叶百部碱和百部新碱)对叶百部组成的百部汤均可显著降低羟脯氨酸含量、减少肺组织胶原沉积,且其影响程度无明显区别,说明不同主成分对叶百部为君药组成的百部汤均能够明显改善博来霉素诱导的小鼠肺纤维化,其作用机制及物质基础有待进一步研究。This study aimed to investigate the protective effects of Baibu Tang on bleomycin-induced pulmonary fibrosis in mice.After intratracheally giving bleomycin(3.5 mg/kg),mice were orally administered Baibu Tang once a day for 14 consecutive days,takingnintedanib as a positive control.The anti-fibrotic effects were assessed by the hydroxyproline level and the histopathological changes in H&E or Masson stained lung tissues.The results revealed that the lung coefficient,hydroxyproline content,inflammation and collagen deposition were increased significantly in the lung tissue of the model mice.Both ethanol and water extracts of Baibu Tang significantly improved all the pathological indexes in mice,but the effect of the ethanol extract was better than that of the water extract.Baibu Tang with Baibu(root of Stemona tuberosa)containing different components(neotuberostemonine,tuberostemonine and stemoninine,respectively)could significantly reduce hydroxyproline level and collagen deposition in the lung tissue of bleomycin-induced mice,and there was no significant difference in their activity.This result showed that the changes in the chemical composition of Stemona tuberosa(Baibu,monarch drug for Baibu Tang)have little effect on the anti-fibrosis activity of Baibu Tang,and its mechanism and material basis need further investigation.

关 键 词：百部汤 肺纤维化 新对叶百部碱 对叶百部碱 百部新碱 

分 类 号：R965[医药卫生—药理学]