松树体细胞胚胎发生技术的发展与应用  

The Development and Application on Pine Somatic Embryogenesis

作　　者：胡继文[1] 郭文冰[1] 邓乐平 钟岁英 王为民 赵奋成[1] 黄婷 吴惠姗[1] 李义良[1] 廖仿炎[1] HU Jiwen;GUO Wenbing;DENG Leping;ZHONG Suiying;WANG Weiming;ZHAO Fencheng;HUANG Ting;WU Huishan;LI Yiliang;LIAO Fangyan(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China;Taishan Hongling Seed Orchard,Jiangmen,Guangdong 529223,China)

机构地区：[1]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520 [2]台山市红岭种子园,广东江门529223

出　　处：《林业与环境科学》2018年第4期152-161,共10页Forestry and Environmental Science

基　　金：广东省科技计划项目(2014A020208032)

摘　　要：植物体细胞胚胎(SE)发生作为上世纪中叶以来最强大的生物技术之一,相比其它无性繁殖方法,具有诸多优势。松树是世界上重要的商品林树种,松树SE研究是开展松树育种与繁育的热门领域。文章综述了松树SE的研究历程,对松属树种的SE发生情况进行了归纳;详细阐述了体胚发生技术在松树新品系选育(包括无性系林业及遗传改良)、苗木扩繁、遗传转化及杂种后代胚拯救方面的应用实例,以期为SE技术在松树上的利用提供参考。Plant somatic embryogenesis(SE),one of the most powerful biotechnologies since the middle of the last century,has many advantages over other methods of asexual reproduction.Pinus spp.is an important commercial forest tree species in the world.SE research of pine trees has become a hot research topic in pine tree breeding and propagation,and SE occurrences of Pinus species and varieties were summarized.This study reviewed the research course of somatic embryogenesis in pine.The application of SE techniques in breeding,including clonal forestry and genetic improvement,genetic transformation of new pine strains and embryo rescue of hybrid progenies were described in detail,which provided references for the use of pine SE technology.

关 键 词：松树 体细胞胚胎发生 无性系林业 遗传转化 胚拯救 

分 类 号：S722.8[农业科学—林木遗传育种]