血清5型和13型副猪嗜血杆菌二价灭活苗的制备和免疫效果评价  

Preparation and Immune Efficacy of Prepared Bivalent Inactivated Vaccine of Serovar 5 and Serovar 13 Against Haemophilus Parasuis

作　　者：朱璐 范小兵 王正皓 代科 文心田 曹三杰 赵勤 文翼平 ZHU Lu;FAN Xiao-bing;WANG Zheng-hao;DAI Ke;WEN Xin-tian;CAO San-jie;ZHAO Qin;WEN Yi-ping(Research Center of Swine Disease/College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院/猪病研究中心,成都611130

出　　处：《四川农业大学学报》2018年第4期514-519,共6页Journal of Sichuan Agricultural University

基　　金：公益性行业(农业)科研专项经费项目(201303034)

摘　　要：【目的】制备一种用于预防副猪嗜血杆菌病的二价灭活苗,并对其免疫效力进行评价。【方法】选择四川地区流行的副猪嗜血杆菌(Haemophilus parasuis,HPS)血清5型MC-203株和血清13型XJ-103株,用甲醛灭活后,经PBS重悬,将2种菌液与佐剂(铝胶)按1:1:2的比例混合制出二价灭活苗。随后用制备的灭活苗免疫仔猪进行相关安全性检验,抗体滴度检测。同时为了对比制备疫苗与国产商品疫苗和进口商品疫苗的免疫效果,进行了仔猪免疫保护效果评价实验。【结果】(1)用0.2%的甲醛在37℃作用20 h可将HPS完全灭活。(2)灭活苗免疫仔猪后未造成不良反应,安全性好。(3)免疫仔猪后14 d即可在体内检测到HPS特异性抗体,196 d的血清中仍可检测到HPS抗体。(4)仔猪免疫保护效力试验表明,所制备的灭活疫苗、进口商品疫苗和国产商品疫苗的免疫保护率分别为80%、80%、60%。【结论】制备的HPS灭活苗免疫仔猪,可以产生较好的免疫应答,并且能在一定程度上抵御HPS的感染。【Objective】The goal of this study is to prepare a bivalent inactivated vaccine for controlling Haemophilus parasuis infection and evaluate itsimmune efficacy.【Method】HPS serovar 5 strain MC-203 and serovar 13 strain XJ-103 prevalent in Sichuan province were inactivated by 0.2%formaldehyde and then resuspended in PBS,after which,the MC-203,XJ-103 and alhydrogel adjuvant were mixed by 1:1:2 to inoculate piglets for security and antibody titer inspection.This newly prepared vaccine was also compared with imported and domestic inactivated vaccines in their immune protection.【Result】①By the use of 0.2%formaldehyde at 37℃for 20 h,HPS can be completely inactivated.②After the inactivated vaccine was immunized to piglets,no adverse reaction was caused,thus its safety was good.③The specific antibody against HPS could be measured at 14 days after inoculation and last at least for 196 days.④The newly prepared inactivated vaccine,imported inactivated vaccine and domestic inactivated vaccine have a protection of 80%,80%and 60%,respectively.【Conclusion】We can conclude that the bivalent inactivated vaccine prepared in this study presents a desired immune efficacy and a promissing role against HPS infection.

关 键 词：副猪嗜血杆菌 二价灭活苗 抗体滴度 免疫保护效力 

分 类 号：S858.28[农业科学—临床兽医学]