重金属累积对泽兰实蝇幼虫生长发育和保护酶的影响  

Effects of heavy metal on development and protective enzymes in the larvae of Procecidochares utilis Stone(Diptera:Tephritidae)

作　　者：马沙[1,2] 兰明先 鲁武锋 李召波 郝凯强[1] 夏涛 张某[1] 李建一 李梦月[1] 李丽芳 高熹 吴国星[1] MA Sha;LAN Ming-Xian;LU Wu-Feng;LI Zhao-Bo;HAO Kai-Qiang;XIA Tao;ZHANG Mou;LI Jian-Yi;LI Meng-Yue;LI Li-Fang;GAO Xi;WU Guo-Xing(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Zhaoyang Plant Protection and Quarantine Station,Zhaotong 657000,Yunnan Province,China)

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南省昭通市昭阳区植保植检站,云南昭通657000

出　　处：《环境昆虫学报》2018年第4期866-872,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金(31460491;31501706)

摘　　要：为了弄清重金属通过寄主植物紫茎泽兰累积后对专性寄生天敌泽兰实蝇的生理影响,采用模拟Cd^(2+)、Pb^(2+)或Zn^(2+)污染的土壤培养法胁迫紫茎泽兰,让泽兰实蝇低龄幼虫寄生取食,利用原子吸收分光光度法测定泽兰实蝇老熟幼虫中Cd^(2+)、Pb^(2+)或Zn^(2+)的含量,同时测定分析泽兰实蝇老熟幼虫的体长、鲜重、能量物质和保护酶的变化。结果表明,实验处理的Cd^(2+)、Pb^(2+)或Zn^(2+)均可通过紫茎泽兰传递并累积到泽兰实蝇老熟幼虫体内,引起老熟幼虫一系列生理变化:长度和鲜重降低;总糖、总蛋白和总脂的含量降低,相应的热量值也降低;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等保护酶的活性降低。本研究为进一步阐明重金属污染对食物链各环节的影响提供了重要的理论依据。In order to understand the physiological effects of heavy metals accumulated by the host plant Eupatorium adenophorum Spreng on Procecidochares utilis Stone,E.adenophorum was exposed to Cd 2+,Pb 2+or Zn 2+respectively in pot experiments,and was parasitized by the low instar larvae of P.utilis.The contents of Cd 2+,Pb 2+or Zn 2+in the mature larvae of P.utilis were determined by atomic absorption spectrophotometry.Meanwhile,the body length and fresh weight of the mature larvae were measured and analyzed,and the energy substance and protective enzymes in the mature larvae were measured and analyzed.The results showed that Cd 2+,Pb 2+or Zn 2+under experimental concentrations were transferred from E.adenophorum to P.utilis,which brought to physiological changes of mature larvae.The body length and fresh weight of the mature larvae decreased.Meanwhile,the contents of energy substances(sugar,protein and lipid)decreased,and the corresponding heat value also decreases.The activities of protective enzymes(SOD,POD and CAT)decreased in the mature larvae.The findings will provide an important theoretical basis for further clarifying the effects of heavy metal pollution on all aspects of the food chain.

关 键 词：重金属 泽兰实蝇 生长发育 保护酶 

分 类 号：Q965.9[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]