我国北方地区鲤浮肿病毒的流行情况调查与监测分析  

EPIDEMIOLOGICAL INVESTIGATION AND SURVEILLANCE ANALYSIS OF CARP EDEMA VIRUS SOURCED FROM NORTHERN OF CHINA

作　　者：徐立蒲 王小亮 李清[2] 王立新[2] 曹欢 王姝 张文 潘勇 王静波 XU Li-Pu;WANG Xiao-Liang;LI Qing;WANG Li-Xin;CAO Huan;WANG Shu;ZHANG Wen;PAN Yong;WANG Jing-Bo(Beijing Aquaculture Technology Extention Station,Beijing 100176,China;National Fisheries Technical Extension Center,Beijing 100125,China)

机构地区：[1]北京市水产技术推广站,北京100176 [2]全国水产技术推广总站,北京100125

出　　处：《水生生物学报》2018年第5期935-941,共7页Acta Hydrobiologica Sinica

基　　金：2017年农业部水生动物重大疫病监测与防治项目;北京市创新团队-观赏鱼产业创新团队(BAIC03-2017)资助~~

摘　　要：为进一步确认及掌握鲤浮肿病毒(Carp Edema Virus,CEV)目前的流行现状和流行特点,于2017年重点调查了我国北方地区5省市26个正发生或曾经发生"鲤急性烂鳃病"的养殖场,随机监测了9个省市的97家鲤和锦鲤养殖场。样品采用实时荧光定量PCR(Quantitative real time PCR,q PCR)法和套式PCR(Nest PCR)方法进行检测,nest PCR法扩增出的产物进行测序和基因分析。结果重点调查的26家养殖场中有20个为CEV阳性,1个为锦鲤疱疹病毒(Koi Herpes Virus,KHV)阳性,1个为孢子虫感染。随机调查的97家养殖场中有50家为CEV检测阳性,阳性率高达52%;可测序的CEV毒株均属于GenogroupⅡa型;样品按不同地区、不同采样水温、不同品种、不同规格鱼等划分,各组均有CEV阳性检出,但检出率均无显著差异。以上结果表明CEV感染是多省市"鲤急性烂鳃病"暴发的主要病因。另外,该病毒病的流行水温在12—27℃。In 2017,to further confirm the speculation and epidemiological traits of carp edema virus(CEV),26 carp farms in 5 provinces of north China,where“acute rot gills disease of carp”,were occurred,surveyed,and sampled.CEV and koi herpes virus(KHV)were detected using quantitative polymerase chain reaction(qPCR)and nested PCR techniques.In addition,97 carp farms in 9 provinces of China were randomly surveyed for CEV and PCR products for sequencing and analyzing viral DNA of CEV.The results showed that 20 farms were CEV-positive samples,1 farm was KHV-positive sample,and 1 farm was infected by Sporozoa among 26 farms in north China.Besides,50 farms were CEV-positive samples among 97 randomly surveyed farms,and infected strains belonged to genogroup II(GII).CEV was detected in different study regions,temperature of water,size of fish,and species of carp with high rate of infection,and there were no significant differences between them.In summary,CEV infection was found to be the major cause of“acute gill-rot disease of carp”in several provinces,and showed high-rate of positive detection in carp farms in north of China.In addition,CEV infection affected various species of common carps including koi carp with different sizes at the temperature range of 12-27℃.

关 键 词：鲤 锦鲤 鲤浮肿病毒 流行情况 监测 

分 类 号：S941.4[农业科学—水产养殖]