α-酮戊二酸对氨氮胁迫下草鱼鳃Na^+/K^+-ATP酶活性及血液生化指标的影响  

EFFECTS OF α-KETOGLUTARATE ON GILL NA^+/K^+-ATPASE ACTIVITY AND BLOOD BIOCHEMICAL PARAMETERS OF GRASS CARP EXPOSURE TO AMMONIA

作　　者：赵玉蓉[1] 付莹[1] 王红权[1,2] ZHAO Yu-Rong;FU Ying;WANG Hong-Quan(College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China;Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province,Changde 415000,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]水产高效健康生产湖南省协同创新中心,常德415000

出　　处：《水生生物学报》2018年第5期996-1002,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金面上项目(31470132);湖南省自然科学基金面上项目(14JJ4039);湖南省发改委试验项目([2013]766)资助~~

摘　　要：试验旨在研究饲粮添加α-酮戊二酸(α-ketoglutarate,α-KG)对氨氮胁迫下草鱼(Ctenopharyngodon idellus)鳃Na^+/K^+-ATP酶活性及血液生化指标的影响。选取初始体重为(24.79±0.11)g的健康草鱼,随机分为3个处理组(对照组,养于曝气后氨氮浓度为1.51 mg/L的自来水中并饲喂基础饲粮;氨氮组,养于氨氮浓度为18.37 mg/L的水中并饲喂基础饲粮;α-KG组,养于氨氮浓度为18.37 mg/L的水中并饲喂添加0.75%α-KG的饲粮)。每处理组设3个重复,每个重复30尾鱼,养殖试验为期42d,分别于第1、第14、第28和第42天采样。结果表明:在饲料中添加α-KG能够有效缓解氨氮胁迫导致的草鱼血浆氨含量(1d)、谷草转氨酶(Aspartate transaminase,AST)活性(14d)、碱性磷酸酶(Alkaline phosphatase,ALP)活性(28d)的显著升高和鳃Na^+/K^+-ATP酶活性(28d)、血浆谷丙转氨酶(Alanine aminotransaminase,ALT)(28d和42d)活性、血浆尿素(UREA)含量(28d)的显著降低,显著增加氨氮胁迫下草鱼血浆球蛋白(Globulin,GLB)含量(28d)。即饲粮α-KG的适量添加能够有效缓解草鱼氨氮胁迫所致的血氨含量升高,维持氨氮胁迫下草鱼鳃Na^+/K^+-ATP酶、血浆谷丙转氨酶、谷草转氨酶、碱性磷酸酶的活性和血浆球蛋白、尿素含量的稳定,从而有利于草鱼缓解氨氮胁迫。A 42-day feeding trial was conducted to investigate the effects ofα-ketoglutarate(α-KG)on gill Na^+/K^+-ATPase activity and blood biochemical parameters of grass carp,Ctenopharyngodon idellus,exposure to ammonia.A total of 270 individuals[initial body weight:(24.79±0.11)g]were randomly assigned to 9 tanks,and these tanks were divided into three groups(Ⅰ:treated with 1.51 mg/L of aerated water and basal diet;Ⅱ:treated with 18.37 mg/L of ammonia solution and basal diet;Ⅲ:treated with 18.37 mg/L of ammonia solution and 0.75%α-KG added diet),with three replicates per each group(n=30).Experimental fish were sampled on 1st,14th,28th,and 42 days,respectively.The results showed that:(1)dietα-KG could effectively alleviate a significant increase in the content of plasma ammonia on the 1st day,the activity of plasma aspartate transaminase on 14th day and plasma alkaline phosphatase on 28th day,which were caused as being exposure to ammonia;dietα-KG could effectively alleviate a significant decrease in the activity of gill Na^+/K^+-ATPase on 28th day,plasma alanine aminotransferase on 28th and 42th days,and the content of plasma urea on 28th day because of being exposure to ammonia.Dietα-KG could significantly increase plasma globulin content on 28th day exposure to ammonia.In conclusion,dietaryα-KG could alleviate the increase of plasma ammonia content exposure to ammonia,and maintain the stabilization in the activity of gill Na^+/K^+-ATPase,plasma alanine transaminase(ALT),plasma aspartate aminotransferase(AST),plasma alkaline phosphatase(ALP),and the content of GLB,as well as urea in grass carp exposure to ammonia.

关 键 词：草鱼 Α-酮戊二酸 氨氮胁迫 Na^+/K^+-ATP酶 血液生化指标 

分 类 号：S963.7[农业科学—水产养殖]