彩色棉多药和有毒化合物输出蛋白MATE家族基因的鉴定及表达分析  

Identification and Expression Analysis of Multidrug and Toxic Compound Extrusion Protein Family Genes in Colored Cotton

作　　者：王作敏 刘瑾 孙士超 张新宇[2] 薛飞[2] 李艳军[2] 孙杰[2] WANG Zuo-Min;LIU Jin;SUN Shi-Chao;ZHANG Xin-Yu;XUE Fei;LI Yan-Jun;SUN Jie(College of Life Sciences,Shihezi University,Shihezi 832003,Xinjiang,China;Agricultural College/Key Laboratory of Oasis Eco-agriculture Xinjiang Production and Construction Group,Shihezi University,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832003

出　　处：《作物学报》2018年第9期1380-1392,共13页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(U1303281);石河子大学动植物育种重点项目(YZZX201601;gxjs2014-yz08)资助~~

摘　　要：植物多药和有毒化合物输出家族(multidrug and toxic compound extrusion,MATE)是一类可转运阳离子染料、氨基葡糖、多种抗生素与药物等次生代谢产物的转运蛋白家族。本研究利用生物信息学手段从陆地棉基因组数据库中鉴定了MATE家族基因,并从基因的系统进化关系、染色体分布、基因结构和表达模式等方面对该基因家族特征进行了比较分析。共鉴定出91个陆地棉MATE基因,命名为Gh MATE1~Gh MATE91。陆地棉MATE蛋白与拟南芥MATE蛋白均可分为A、B、C、D、E、F和G 7个亚家族,其中84个Gh MATE蛋白具有12个典型的跨膜结构域。染色体定位显示,Gh MATE家族成员定位在不同的25条染色体上,共形成5个基因簇。q RT-PCR分析发现,Gh MATE家族基因在棉花各组织中均有表达,但表达模式各不相同,其中Gh MATE13和Gh MATE23在棕色棉纤维中的表达量明显高于在白色棉中,表明它们可能与棕色棉纤维的颜色形成相关。本研究为进一步解析棉花MATE家族基因的功能和作用机制积累了有价值的资料。MATE(multidrug and toxic compound extrusion)is a transport proteins family that can transport toxins,glucosamine,antibiotics and drugs.In this study,the MATE family genes were identified from genome database of Gossypium hirsutum L.through bioinformatics analysis and phylogenetic relationship,chromosome distribution,gene structure and expression patterns of this family were comprehensively compared.A total of 91 GhMATE genes were found in the cotton genome and named as GhMATE1–GhMATE91.MATE genes of cotton were classified into seven groups namely A,B,C,D,E,F,and G with a classification is consistent with Arabidopsis.Multiple sequence alignment and conserved domain prediction indicated that 84 of the 91 GhMATE proteins contained 12 typical transmembrane domains.The chromosome mapping analysis showed that GhMATE genes were distributed with different densities over 25 chromosomes and clustered into five clusters.The qPCR showed that the GhMATE genes expressed in all tissues of cotton with different expression patterns.GhMATE13 and GhMATE23 were preferentially expressed in brown cotton fibers than in white cotton fibre,suggesting that they may play an important role in brown color formation of cotton fibre.This study provides valuable informations for dissecting functions and molecular mechanisms of MATEs in cotton.

关 键 词：陆地棉 彩色棉 MATE家族 生物信息学 实时荧光定量PCR 

分 类 号：S562[农业科学—作物学]