高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能  

Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein

作　　者：贺茂芳 张博[1] 唐一梅[1] 韩禄[1] HE Mao-Fang;ZHANG Bo;TANG Yi-Mei;HAN Lu(College of Pharmacy,Institute of Drug Research,Xi′an Medical University,Xi′an 710021,China)

机构地区：[1]西安医学院药学院,西安医学院药物研究所,西安710021

出　　处：《分析化学》2018年第9期1486-1492,共7页Chinese Journal of Analytical Chemistry

基　　金：本文系西安医学院博士科研启动基金(No.2016D0C06)和国家自然科学基金孵育项目(No.2017 GJFU08)资助。

摘　　要：为了获得高容量的阴离子交换吸附剂,本研究以Fe_3O_4磁性纳米粒子为基质,在其表面包覆聚多巴胺,然后与树枝状大分子聚乙烯亚胺反应,制得多氨基化磁性微球。此微球的离子交换容量为9.1 mmol/g。采用透射电镜、红外光谱和热重分析对材料进行了表征。以β-酪蛋白和牛血清蛋白(BSA)为模型蛋白,采用静态吸附法研究了此微球对蛋白质的吸附性能,在最佳条件下测定了微球对蛋白质的静态吸附等温线。结果表明,此微球对蛋白质的最佳吸附时间为2h,缓冲溶液的最佳pH值为7.0。在此条件下,对β-酪蛋白和BSA的最大吸附容量分别为237.5和204.5μg/mg,而对溶菌酶和核糖核酸酶A几乎不产生吸附。实验结果表明,此微球对酸性蛋白具有选择性,在蛋白质分离纯化方面具有良好的应用前景。A high capacity anion exchange magnetic absorbent was prepared by immobilization of hyperbranched polyethyleneimine(PEI)on polydopamine-coated Fe 3O 4 microspheres(Fe 3O 4@pDA)and the ion exchange capacity was determined as 9.1 mmol/g.The absorbent was characterized by transmission electron microscope(TEM),infrared spectroscopy(IR)and thermogravimetric analysis(TGA).Moreover,the adsorption behavior of the absorbent for protein was carefully investigated by static adsorption using bovine serum albumin(BSA)andβ-casein as models and then the static isotherms were determined under the optimum conditions.It was shown that the highest adsorption amount for protein was obtained when the adsorption time was 2 h and the pH was 7.0.Under the optimum conditions,the saturated adsorption capacity towardsβ-casein and BSA was determined as high as 237.5 and 204.5μg/mg,respectively.Comparatively,the adsorbent exhibited little adsorption towards lysozyme and Rnase A,showing highly selectivity towards acidic protein.Therefore,the adsorbent exhibited potential in the separation and purification of protein from real samples.

关 键 词：高容量 阴离子交换 聚乙烯亚胺 蛋白质 分离纯化 磁性微球 

分 类 号：O652.63[理学—分析化学] TQ424[理学—化学]