新疆石河子月季花附生酵母菌多样性分析  

Diversity analysis of the epiphytic yeasts in the flowers of Rosa chinensis Jacq. from Shihezi, Xinjiang

作　　者：马雪 孙淑文 李杨[1] 孙燕飞[1] 雷勇辉[2] MA Xue;SUN Shu-wen;LI Yang;SUN Yan-fei;LEI Yong-hui(College of Life Sciences,Shihezi University,Shihezi 832003,China;Agricultural College,Shihezi University,Shihezi 832003,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]石河子大学农学院,新疆石河子832003

出　　处：《广东农业科学》2018年第8期43-49,173,共8页Guangdong Agricultural Sciences

基　　金：新疆特色植物基因资源研究与利用创新团队专项(2014CC005);国家重点研发计划典型脆弱生态修复与保护研究重点专项(2016YFC0501404)

摘　　要：以新疆石河子地区月季花为试材,采用Illumina(Mi Seq)平台进行26S r DNA高通量测序分析、α多样性分析、可培养酵母菌的分离纯化技术(稀释涂布法和孢子弹射法)、26S r DNA D1/D区测序鉴定、糖发酵及碳同化等生理生化实验,研究了月季花上附生酵母菌(可培养和不可培养)菌群多样性,并对系统进化进行分析,以期为有效开发利用新疆酵母菌资源奠定基础,并为探索花蜜微生物与植物相互作用提供理论依据。结果表明:经26S r DNA高通量测序筛选后得到41 337个优化序列,划分入3 078个OTU,月季附生酵母菌群落结构丰富,鉴定为3个门、10个纲、13个目、22个科、37个属、90个种。担子菌门(Basidiomycota)为优势门、占18.9%,线黑粉菌属(Filobasidium)为优势属、占12.2%,酵母菌种群的丰度存在差异;可培养的酵母菌共鉴定出3个酵母菌属4个已知种群,分别为季也蒙毕赤酵母(Meyerozyma guilliermondii)、粘性红酵母(Rhodotorula mucilaginosa)、乌兹别克斯坦隐球酵母(Cryptococcus uzbekistanensis)、浅白隐球酵母(Cryptococcus albidus),优势酵母属为隐球酵母属(Cryptococcus)。Using the Rosa chinensis Jacq.in Shihezi area of Xinjiang as the material,the Illumina(MiSeq)platform was used to carry out 26S rDNA high throughput sequencing analysis and Alpha diversity analysis.The experiment made use of isolation and purification techniques of culturable yeast(dilution coating method and spore ejection method),sequencing and identification of 26S rDNA D1/D2 region,carbohydrate fermentation tests,carbon assimilation tests and related physiological tests.The diversity and phylogenetic analysis of epiphytic yeast(culturable and non culturable)on R.chinensis Jacq.aimed at laying a foundation for the effective utilization of Xinjiang yeast resources.It also provided a theoretical basis for exploring the interaction between nectar microorganisms and plants.The results showed that 41 337 optimized sequences were selected by high throughput sequencing of 26S rDNA and divided into 3 078 OTU(Operational Taxonomic Unit).The epiphytic yeast community structure was diverse.Then the epiphytic microorganism community structure was identified as 3 phylum,10 classes,13 orders,22 families,37 genera and 90 species.The abundance of yeast population were different.Basidiomycota was dominant phylum,which was accounted for 18.9%,among the yeast community.Filobasidium was dominant genera,which was accounted for 12.2%.4 species culturable yeasts from 3 genera were recognized,including Meyerozyma guilliermondii,Rhodotorula mucilaginosa,Cryptococcus uzbekistanensis and Cryptococcus albidus.The dominant yeast genera is Cryptococcus.

关 键 词：新疆石河子 月季 酵母菌多样性 26S RDNA D1/D2 高通量测序 

分 类 号：S685.12[农业科学—观赏园艺] Q939.5[农业科学—园艺学]