超声-微波协同萃取法提取桑叶γ-氨基丁酸的工艺研究  

Study on the microwave-ultrasonic assisted extration process of mulberry leaf γ-aminobutyric acid

作　　者：钟杨生[1] 陈恒文[1] 陈芳艳[1] 林健荣[1] ZHONG Yang-sheng;CHEN Heng-wen;CHEN Fang-yan;LIN Jian-rong(College of animal science,South China Agricultrual University,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《广东农业科学》2018年第8期107-112,共6页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(201403064);广东省科技计划项目(2017A020208044)

摘　　要：桑叶尤其是桑嫩芽中富含γ-氨基丁酸(γ-aminobutyric acid,GABA),为开发利用桑叶GABA,采用超声-微波协同萃取法提取桑叶GABA。通过单因素和Box-Behnken试验,探究提取时间、料液比、提取温度、超声波功率和微波功率对桑叶GABA提取量的影响。结果表明,提取时间、料液比及提取温度对桑叶GABA的提取影响较大,超声-微波协同萃取法提取的最佳技术参数为料液比1∶34、微波功率为144 W、超声波功率为50 W、提取时间为165 s,在此工艺条件下GABA提取量为5.83 mg/g。可见,采用超声-微波协同萃取法提取桑叶GABA具有提取率高、提取时间短等优点。Mulberry leaf,expecially tender shoot,is rich inγ-aminobutyric acid(GABA).In order to develop and utilize mulberry leafγ-aminobutyric acid(GABA),mulberry leaf GABA was extracted from mulberry leaves using pulsed ultrasonic and microwave-assisted extraction method.In this study,the effects of extration time,material-solvent ratio,extraction temperature,ultrasonic power and microwave power on extraction yield were investigated through single-factor experiment and box-behnken experiment.The results of single-factor experiment and Box-Behnken test indicated that the extraction time,material and liquid ratio and extraction temperature had a greater influence on the extraction of GABA from mulberry leaves.The best parameters of ultrasonic-microwave collaborative extraction were:material-solvent ratio 1(g):34(mL),microwave irradiation power at 144 W,ultrasonic power at 50 W,and extraction time at 165 s.With this extraction condition,GABA yield was 5.83 mg/g.This method has the advantages of high extraction rate and short extraction time.

关 键 词：桑叶 Γ-氨基丁酸 超声-微波协同萃取 Box-Behnken响应面设计 

分 类 号：S888.2[农业科学—特种经济动物饲养] TS201.1[农业科学—畜牧兽医]