微图案技术及在组织工程中的应用  

Micro-pattern technique and its application in tissue engineering

作　　者：邱昌俊 浑婷婷 赵莹彤 赵峰[1,3] 何静雯 孙艳[1,2,3] Qiu Chang-jun;Hun Ting-ting;Zhao Ying-tong;Zhao Feng;He Jing-wen;Sun Yan(Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China;Beijing Advanced Innovation Centre for Biomedical Engineering,Beihang University,Beijing 102402,China;State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China)

机构地区：[1]北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室,北京市100191 [2]生物医学工程高精尖创新中心,北京航空航天大学,北京市102402 [3]中国科学院上海微系统与信息技术研究所,传感技术联合国家重点实验室,上海市200050

出　　处：《中国组织工程研究》2018年第30期4900-4905,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(31470942)。

摘　　要：背景:随着微图案技术的不断创新和发展,其在细胞生物学、组织工程和再生医学等相关研究中也有着更加广泛的应用,为体外构建人工组织提供了新的动力。目的:总结并讨论微图案技术的分类、特点及其在组织工程中的应用。方法:检索2001至2017年Web of Science数据库、Pub Med数据库及中国知网与微图案技术及其在组织工程中应用相关的文献,英文检索词为"micro-pattern technique,tissue engineering",中文检索词为"微图案技术,组织工程"。结果与结论:通过微图案技术可形成图案化的细胞排列,进而控制干细胞的形状,调控干细胞融合和分化等,以形成可用于组织替代物的人工组织工程产品,被广泛应用于骨组织工程、肌肉组织工程、血管组织工程及神经组织工程及肝脏组织工程研究中。然而,利用微图案技术构建人工组织的过程中,仍有许多细胞生物学机制和内在机制亟待进一步探索和阐明,例如:如何通过微图案技术构建人工肌肉组织,如何纯化许旺细胞,以及进一步促进微图案技术在血管组织工程和肝脏组织工程中的发展和创新等问题。BACKGROUND:With the constant innovation and development of micro-pattern technology,it has also been applied widely in cell biology,tissue engineering and regenerative medicine,which provides new impetus for the construction of artificial tissues in vitro.OBJECTIVE:To summarize the classification,characteristics and application of micro-pattern technique in tissue engineering.METHODS:The authors performed a data retrieval of PubMed,Web of Science and CNKI to search the articles addressing micro-pattern technique and its application in tissue engineering published from 2001 to 2017,and reviewed the literatures systematically.The keywords were“micro-pattern technique,tissue engineering”in English and Chinese,respectively.RESULTS AND CONCLUSION:Using the micro-pattern technique,patterned cell alignment is formed to control the shape of stem cells,and regulate stem cell fusion and differentiation.Moreover,artificial tissue engineering products have been developed and used as tissue substitutes for bone tissue engineering,muscle tissue engineering,vascular tissue engineering,nerve tissue engineering and liver tissue engineering.However,cell biology mechanisms and internal mechanisms underlying the use of micro-pattern technique in the artificial tissue construction need to be further explored and elucidated.For example,future investigations are warranted on how to construct artificial muscle tissue using the micro-pattern technique,how to purify Schwann cells,as well as the development and innovation of micro-pattern technique in vascular and liver tissue engineering research.

关 键 词：细胞图案化 细胞形状 细胞分化 微图案技术 组织工程 细胞外基质 3D打印技术 纤维支架 生物材料 细胞外基质 细胞分化 组织工程 

分 类 号：R318[医药卫生—生物医学工程]