盾壳霉对2种除草剂的敏感性评价  

Sensitivity evaluation of Coniothyrium minitans to glyphosate and glufosinate

作　　者：强翠翠 程家森[1,2] 谢甲涛[1,2] 付艳苹[2] 姜道宏[1,2] QIANG Cuicui;CHENG Jiasen;XIE Jiatao;FU Yanping;JIANG Daohong(State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China;The Provincial Key Laboratory of Crop Diseases Monitoring and Safety Control,Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070 [2]华中农业大学湖北省作物病害监测和安全控制重点实验室,武汉430070

出　　处：《华中农业大学学报》2018年第5期25-31,共7页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2017YFD0200600);中央高校基本科研业务费专项(2662015PY117);油菜现代产业技术体系岗位科学家科研专项(CARS-13)

摘　　要：为了与播前除草措施相配合,达到油菜轻简化栽培的目的,开展了盾壳霉对田间常用灭生型除草剂草甘膦和草铵膦敏感性的研究。结果发现,草甘膦和草铵膦原药对盾壳霉菌丝生长有较强抑制作用,EC50分别为714.3μg/mL和574.5μg/mL;草甘膦对盾壳霉孢子萌发抑制作用较弱,EC50为1 019.8μg/mL,而草铵膦原药对孢子的萌发基本没有抑制作用;2种除草剂原药均抑制核盘菌菌丝生长,EC50分别为1 695.8μg/mL和223.5μg/mL;2种除草剂商品制剂对盾壳霉孢子没有杀灭作用,但延迟孢子的萌发,影响盾壳霉对核盘菌菌核的寄生。研究结果表明,在田间生产中盾壳霉可以与草甘膦和草铵膦2种除草剂进行混合施用。Coniothyrium minitans is a parasitic fungus of Sclerotinia sclerotiorum,and has been developed into biological agent to control diseases caused by S.sclerotiorum.To apply the agent with the nonselective herbicides glyphosate and glufosinate to the soil before seeding,in this study,the sensitivity of C.minitans strain ZS-1 to glyphosate and glufosinate was evaluated.Both glyphosate and glufosinate had inhibitory effects on the mycelial growth of strain ZS-1,with EC 50 of 714.3μg/mL and 574.5μg/mL,respectively.The inhibition of spore germination of glyphosate was much weak(EC 50 1 019.8μg/mL),while glufosinate had no effect on spore germination.Both herbicides could strongly inhibit the mycelial growth of S.sclerotiorum strain 1980 with stronger inhibition of glufosinate(EC 50 223.5μg/mL)than glyphosate(EC 50 1 695.8μg/mL).In the presence of both herbicides,parasitism of strain ZS-1 to sclerotia was reduced.Considering the characteristics of glyphosate and glufosinate,C.minitans could be used to the soil before seeding with the herbicides.

关 键 词：盾壳霉 核盘菌 草甘膦 草铵膦 敏感性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]