黄金茶多糖结构初探及除蛋白工艺研究  

Structural Characterization of Gold tea Polysaccharide and Its Deproteinization Pprocess

作　　者：熊磊 陈慧 王宁 刘鑫 李祥格 吴国强 李景恩 王文君 XIONG Lei;CHEN Hui;WANG Ning;LIU Xin;LI Xiang-ge;WU Guo-qiang;LI Jing-en;WANG Wen-jun(Key Laboratory for Agricultural Product Processing and Quality Control of Nanchang City,College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学食品科学与工程学院/南昌市农产品加工与质量控制重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2018年第4期817-824,共8页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金资助项目(31560459);江西省自然科学基金项目(20151BAB204037);江西省高等学校科技落地计划资助项目(KJLD13027);江西省教育厅科技计划项目(GJJ13281);广东省农产品加工重点实验室开放基金(201603);江西省研究生创新专项资金项目(YC2016-S186)~~

摘　　要：试验以黄金茶为原料,通过超声波法提取黄金茶粗多糖。粗多糖经除脂肪、色素、蛋白等杂质,以0.3%Na Cl为洗脱剂经DEAE-纤维素柱纯化后得到纯度为65.4%的黄金茶多糖。利用紫外光谱(UV)、红外光谱(FT-IR)、气相色谱(GC)、扫描电镜(SEM)初步对其结构进行探究;通过苯酚-硫酸法测定多糖含量,考马斯亮蓝法测定蛋白质含量。采用Sevage法对黄金茶多糖进行除蛋白工艺研究,在单因素试验基础上进行L9(33)正交试验,确定最佳脱蛋白工艺条件。结果表明:黄金茶多糖含有部分糖蛋白,含有#OH、#CH、#COOH、S=O、#C=O等官能团;黄金茶多糖由鼠李糖(Rha)、阿拉伯糖(Ara)、木糖(Xyl)、甘露糖(Man)、葡萄糖(Glu)、半乳糖(Gal)6种单糖组成,且6种单糖的摩尔比为0.069∶0.148∶0.056∶0.051∶0.566∶0.102。黄金茶多糖的最佳除蛋白工艺条件为:Sevage试剂添加量为多糖溶液体积的1/3,振摇时间为20 min,氯仿与正丁醇体积比为4∶1。In this experiment,gold tea was used as the experimental raw material,and ultrasonic assisted extraction method was applied to extract the crude polysaccharide from gold tea.After removal of fat,protein,pigment and other impurities,the polysaccharide was preliminary separated using DEAE cellulose column and 0.3%NaCl elution,and the purity of gold tea polysaccharide was 65.4%.The structure of gold tea polysaccharide was characterized with different methods such as ultraviolet spectroscopy,infrared spectroscopy(FT-IR),gas chromatography(GC)and scanning electron microscopy(SEM).The polysaccharide content was determined with the phenol-sulfuric acid method,and the protein content was determined with the coomassie brilliant blue method.The sevage method was used to remove protein from gold tea polysaccharide.Single factor tests were used to study the deproteinization efficiency of gold tea polysaccharide.Orthogonal experiment L 9(3 3)was designed to determine the optimal conditions of deproteinization.The results showed that gold tea polysaccharide contained some glycoproteins.Gold tea polysaccharide contains-OH,-CH,-COOH,S=O,-CO and other functional groups.Gold tea polysaccharide contains rhamnose(Rha),arabinose(Ara),xylose(Xyl),mannose(Man),glucose(Glu)and galactose(Gal),and the molar ratio of the six monosaccharides was 0.069∶0.148∶0.056∶0.051∶0.566∶0.102.The optimal conditions for deproteinization of gold tea polysaccharide were as follows:the dosage of sevage reagent was 1/3 volumes of the polysaccharide solution,the volume ratio of chloroform to n-butanol was 4∶1,and the shaking time was 20 min.

关 键 词：黄金茶 多糖 结构初探 除蛋白 

分 类 号：TS271[轻工技术与工程—农产品加工及贮藏工程] TQ539[轻工技术与工程—食品科学与工程]