血管紧张素Ⅱ2型受体对大鼠子宫内膜异位症血管生成的影响  

The Role of Angiotensin Ⅱ Type 2 Receptor on Angiogenesis in Rats with Endometriosis

作　　者：张真真 戴姝艳 ZHANG Zhenzhen;DAI Shuyan(Department of Obstetrics and Gynecology,Shengjing Hospital,China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2018年第9期792-796,共5页Journal of China Medical University

基　　金：留学回国人员科研启动基金(教外司留[2009]8号)

摘　　要：目的探讨血管紧张素Ⅱ2型受体(AT2R)对大鼠子宫内膜异位症血管生成的影响。方法 Vernon自体移植法建立大鼠子宫内膜异位症模型,造模成功后,随机分为实验组(AT2R激动剂CGP42112A 5、15、30μg·kg^(-1)·d^(-1)),阳性对照组(AT1R阻滞剂氯沙坦10 mg·kg^(-1)·d^(-1))和阴性对照组(无菌注射用水),分别给药处理。4周后处死大鼠,比较治疗后异位结节体积差异,免疫组化检测异位结节组织中微血管密度(MVD),ELISA法检测大鼠血清中血管内皮生长因子(VEGF)及肿瘤坏死因子α(TNF-α)的表达。结果与对照组相比,CGP42112A可剂量依赖性的缩小异位结节体积,降低异位结节的MVD(P<0.01);大鼠血清中VEGF及TNF-α表达水平也明显低于对照组(P<0.05)。结论 AT2R能够明显抑制大鼠子宫内膜异位症血管的生成,为子宫内膜异位症的靶向治疗提供了新的方向。Objective To investigate the effects of angiotensinⅡtype 2 receptor(AT2R)on the inhibition of angiogenesis in rats with endometriosis(EMs).Methods The rat model of endometriosis was established by the Vernon autologous transplant method.Rats with endometriosis were randomly divided into the experimental group(AT2R agonist CGP42112,5,15,and 30μg·kg-1·d-1),control group(AT1R antagonist losartan,10 mg·kg-1·d-1),and placebo group(sterile water for injection).The volume of the ectopic foci was measured after four weeks,followed by the collection of the abdominal aortic and ectopic foci.The microvessel density(MVD)was assessed by immunohistochemcial analysis of CD34 expression.Serum concentrations of tumor necrosis factorα(TNF-α)and vascular endothelial growth factor(VEGF)were measured by ELISA.Results Compared to the control group,the experimental group showed ectopic foci atrophy promotion and a CGP42112 dose-dependent reduction in the MVD of the ectopic foci(P<0.01).Serum concentrations of TNF-αand VEGF expression were significantly lower in the CGP42112A groups than those in the control group(P<0.05).Conclusion AT2R can significantly inhibit the angiogenesis of rats with EMs,and EMs targeted therapy may be a new research direction.

关 键 词：子宫内膜异位症 血管紧张素Ⅱ2型受体 微血管密度 血管生成 

分 类 号：R71[医药卫生—妇产科学]