IVIM-DWI评估阿霉素诱导大鼠局灶性肾小球硬化的肾损害进程  

Assessing of the progression of renal injury of adriamycin induced focal segmental glomerulosclerosis by IVIM-DWI in rat models

作　　者：王金璇 吴焕焕[1] 孙浩然[1] WANG Jin-xuan;WU Han-han;SUN Hao-ran(Department of Radiology,the General Hospital of Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院放射科,天津300052 [2]渭南市中心医院医学影像科,陕西省714000

出　　处：《放射学实践》2018年第8期800-805,共6页Radiologic Practice

基　　金：国家自然科学基金资助项目(81171316)

摘　　要：目的:探讨多b值体素不相干运动(IVIM)DWI评估阿霉素(ADR)诱导的大鼠局灶性肾小球硬化进程的可行性。方法:15只健康雄性SD大鼠在注射ADR前及注射后2、4、6及8w时分别行多b值DWI检查。利用单指数和双指数模型获得多个扩散相关参数图,包括表观扩散系数(ADC)、流体分数(f)、假性扩散系数(D*)和纯组织扩散系数(D)。在各扩散参数图像上选择双侧肾门层面分别于肾皮质(CO)、外髓外带(OM)和外髓内带(IM)勾画感兴趣区进行参数值的测量。每个时间点随机选取3只大鼠于处死后取出双肾标本进行组织病理学观察。结果:注射ADR后,CO和OM的ADC值在2w时较注药前增加,分别为(6.87±1.30)×10^(-4)和(6.60±1.80)×10^(-4) mm^2/s,随后均呈现下降趋势。CO、OM及IM的f值均呈连续下降趋势,与注药前(0.56±0.08)比较,CO的f值在6w时(0.46±0.14)无显著变化(P>0.05),而在8w时(0.38±0.11)明显降低(P<0.05)。CO和OM的D*值于6w时(8.10±2.46,9.90±4.06)显著升高(P<0.05),而在8w时[(12.87±3.52)×10^(-2)和(13.16±3.86)×10^(-2) mm^2/s]与其它各时间点间的差异均具有统计学意义(P<0.05)。各分带的D值整体呈现先降低然后稍升高的趋势,但变化均较为平稳;CO的D值仅在8w时[(4.16±2.34)×10^(-4) mm^2/s]与正常[(2.65±1.30)×10^(-4) mm^2/s]及2w时[(2.75±0.93)×10^(-4) mm^2/s]的差异有统计学意义(P<0.05)。组织病理学检查证实实验大鼠的肾脏随时间推移逐步出现肾小球硬化及间质纤维化的表现。结论:IVIM-DWI可反映局灶性肾小球硬化时肾实质内微灌注下降和间质纤维化改变。Objective:To evaluate the feasibility of multi-b values intravoxel incoherent motion(IVIM)MR DWI parameters to monitor the progress of adriamycin(ADR)induced focal segmental glomerulosclerosis in rats.Methods:Multi-b values DWI was performed before and 2w,4w,6w and 8w after ADR administration in 15 healthy SD rats.Maps of apparent diffusion coefficient(ADC),fluid fraction(f),pseudodiffusivity(D*)and pure diffusivity(D)were obtained using both mono-and bi-exponential models.The regions of interest(ROI)at renal cortex(CO),outer zone of the outer medulla(OM)and inner zone of the outer medulla(IM)at the hilum level on the four parameters maps were drawn,and then the values were measured.After each MRI acquisition,three rats were selected randomly and scarified at each time point for further histopathological examination.Results:The ADC values of CO and OM[(6.87±1.30)×10^-4 and(6.60±1.80)×10^-4 mm^2/s]increased at the 2 rd week and then decreased after ADR administration.The f values of CO,OM and IM kept decreasing all the times,the change of CO was not significant(P>0.05)until the 6 th week(0.46±0.14)compared to that before ADR injection(0.56±0.08);however,it decreased since then and was obviously below the normal level at the 8 th week(0.38±0.11,P<0.05).The D*values of CO and OM(8.10±2.46 and 9.90±4.06)were increased at the 6 th week with statistic difference(P<0.05),and at the 8 th week the D*values[(12.87±3.52)×10^-2 and(13.16±3.86)×10^-2 mm 2/s]were significantly increased(P<0.05)when compared with those at all the other time points.The D values of the three renal regions decreased at the first two weeks and then slightly increased,the variation were overall steady.Only the D value of CO at the 8 th week[(4.16±2.34)×10^-4 mm^2/s]showed significant difference(P<0.05)compared to that of normal group[(2.65±1.30)×10^-4 mm^2/s]and the 2 rd week[(2.75±0.93)×10^-4 mm^2/s].Histopathological examination showed glomerular sclerosis and interstitial fibrosis.Conclusion:IVIM-DWI can demonstrate the decreas

关 键 词：阿霉素 肾脏疾病 肾小球硬化 表观扩散系数 体素不相干运动 扩散加权成像 实验研究 

分 类 号：R445.2[医药卫生—影像医学与核医学] R692.6[医药卫生—诊断学]