强脉冲光对大鼠Ⅱ度烫伤模型的建立  

Establishment of Deep Ⅱ Degree Scald Model in Rats by Intense Pulsed Light

作　　者：王培森 袁佩雯 李勤雨 张宝林[2] 张春萍[2] 索金荣 钱会利[2] 侯秀英[2] WANG Pei-sen;YUAN Pei-wen;LI Qin-yu;ZHANG Bao-lin;ZHANG Chun-ping;SUO Jin-rong;QIAN Hui-li;HOU Xiu-ying(Shanxi Medical University First Clinical Medical College,Taiyuan 030001,China;First Hospital of Shanxi Medical University,Taiyuan 030000,China)

机构地区：[1]山西医科大学第一临床医学院,太原030001 [2]山西医科大学第一医院,太原030000

出　　处：《科学技术与工程》2018年第22期174-177,共4页Science Technology and Engineering

基　　金：山西省重点研发计划(社会发展方面)(201603D321080)资助.

摘　　要：为探索脉冲光对大鼠深Ⅱ度烫伤模型建立的实验条件,将24只大鼠随机分成四组,每组6只,选取大鼠脊柱两侧皮肤作为烫伤部位。第1组为正常对照组,第2、3、4组烫伤条件分别为强脉冲光照射7次、8次和9次,每次能量为20 J/cm^2。建立模型后并进行组织形态学检查。结果表明:第2组大鼠为Ⅰ度烫伤,第3组大鼠为深Ⅱ度烫伤,第4组为Ⅲ度烧伤。大鼠模型制作过程中,烫伤的程度同时受照射次数、能量的影响。实验中所建立的大鼠深Ⅱ度烫伤模型制备方法可以通过控制光照强度和次数,利用强脉冲光建立模型操作简单,制造的损伤程度、损伤面积均匀,重复性好。To explore the experimental conditions of intense pulsed light on deep second degree scald in rats,twenty-four rats were randomly divided into four groups with 6 rats in each group.The skin on both sides of the spine was selected as the scald site.The first group was normal control group,the second,third and fourth group scald conditions were intense pulsed light irradiation 7 times,8 times and 9 times,each energy 20 J/cm 2,the model was established and histomorphological examination.The results show that the rats in group 2 were scalded for degreeⅠ,the rats in group 3 were deep II degree scalds,and the rats in group 4 wereⅢdegree burns.In the process of rat model making,the degree of scald is affected by the number of irradiation and the energy at the same time.The deep II degree scald rat model established can control the light intensity and the number of times,the use of pulsed light to establish the model is simple,the degree of damage made,the damage area is uniform,good repeatability.

关 键 词：烫伤 脉冲光 模型 苏木素-伊红 

分 类 号：R644[医药卫生—外科学]