抵抗素样分子影响大鼠主动脉壁血管平滑肌细胞收缩作用的机制  

Contraction of RELM on smooth muscle cells of aortic wall vessels in rats

作　　者：段淑香 张洪强 杨永曜[3] 张红明[2] 徐庆国 DUAN Shuxiang;ZHANG Hongqiang;YANG yongyao;ZHANG Hongming;XU Qingguo(Shandong Provincical Third Hospital,Jinan 250000,China)

机构地区：[1]山东省立第三医院,济南250000 [2]济南军区总医院 [3]贵州省人民医院

出　　处：《山东医药》2018年第33期36-38,42,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81660080)

摘　　要：目的探讨抵抗素样分子(RELMɑ)对大鼠主动脉平滑肌细胞的收缩作用及其机制。方法取大鼠主动脉去内皮血管环,利用Powerlab四道生理仪记录加入RELMα、AngⅡ、肌球蛋白轻链激酶(MLCK)抑制剂及等量平衡液处理前后的血管张力变化。采用贴壁法培养大鼠主动脉平滑肌细胞并分为正常对照组、阳性对照组(1×10^(-7)mol/L AngⅡ)、RELMα刺激组(4×10^(-8)mol/L RELMα)、RELMα+钙调素(CaM)抑制剂组(RELMα+W-7)、RELMα+MLCK抑制剂组(RELMα+ML-7),采用Western blotting法检测各组大鼠主动脉平滑肌细胞CaM、MLCK蛋白表达。结果加入等量平衡液、AngⅡ、RELMα、RELMα+MLCK处理后,血管环血管张力分别为7.27%±2.23%、59.97%±5.56%、85.07%±3.06%、11.02%±2.41%。与加入等量平衡液、AngⅡ比较,加入RELMα后血管张力升高;再加入ML-7后血管张力下降,且低于加入AngⅡ者,差异均有统计学意义(P均<0.05)。大鼠主动脉平滑肌细胞CaM蛋白表达RELMα刺激组(1.41±0.33)高于正常对照组(0.24±0.08)、阳性对照组(0.62±0.20),MLCK蛋白表达RELMα刺激组(1.29±0.30)高于正常对照组(0.23±0.15)、阳性对照组(0.60±0.27),差异均有统计学意义(P均<0.05)。RELMα+CaM抑制剂组CaM蛋白、RELMα+MLCK抑制剂组MLCK蛋白表达分别较相应RELMα刺激组降低(P均<0.05)。结论 RELMα/FIZZ1可引起大鼠主动脉平滑肌细胞收缩,其机制可能与Ca^(2+)-CaM-MLCK途径相关。Objective To investigate the contraction and its mechanism of resistin like moleculeα(RELMα)on rat aortic smooth muscle cells(RASMCs).Methods The vascular ring of endothelium of aorta of rats was taken out.The changes of vascular tension before and after treatment with RELMα,Ang II,myosin light chain kinase(MLCK)and equal balance solution were recorded by the Powerlab four track physiological instrument.The rat aortic smooth muscle cells were cultured and divided into the normal control group,positive control group(1×10-7 mol/L AngⅡ),RELMαstimulation group(4×10-8 mol/L RELMα),RELMα+calmodulin(CaM)inhibitor group(RELMα+W-7),and RELMα+MLCK inhibitor group(RELMα+ML-7).The expression of CaM and MLCK protein in the rat aortic smooth muscle cells was detected by Western blotting.Results After the treatment of equal balance fluid,Ang II,RELMα,and RELMα+MLCK,the vascular tension of vascular ring was 7.27%±2.23%,59.97%±5.56%,85.07%±3.06%and 11.02%±2.41%,respectively.Compared with the addition of equal balance fluid and Ang II,the vascular tension increased after adding RELMα,and the vascular tension decreased after the addition of ML-7,which was lower than that of Ang II,with statistically significant difference(P<0.05).The expression of CaM protein of rat aortic smooth muscle cells in the RELMαstimulation group(1.41±0.33)was higher than that in the normal control group(0.24±0.08)and the positive control group(0.62±0.20),and the MLCK protein expression in the RELMαstimulation group(1.29±0.30)was higher than those in the normal control group(0.23±0.15)and the positive control group(0.60±0.27),with statistically significant difference(all P<0.05).The expression of CaM protein in the RELMα+CaM inhibitor group and the expression of MLCK protein in the RELMα+MLCK inhibitor group was lower than that in the corresponding RELMαstimulation group(all P<0.05).Conclusion RELMα/FIZZ1 can induce contraction of rat aortic smooth muscle cells,which may be related to Ca 2+-CaM-MLCK pathway.

关 键 词：平滑肌细胞 主动脉 抵抗素样分子α 血管环 钙调素 肌球蛋白轻链激酶 大鼠 

分 类 号：R331.3[医药卫生—人体生理学]