金福菇多糖对衰老大鼠p16基因表达影响  

Effect of Jin Fu Mushroom Polysaccharide on p16 Gene Expression in Aging Rats

作　　者：张春军[1] 董凯[1] 董琦[1] 宋洁[1] 曾海 杨国宏[1] ZHANG Chunjun;DONG Kai;DONG Qi(Mudanjiang Medical University,Mudanjiang 157000,China)

机构地区：[1]牡丹江医学院,黑龙江牡丹江157000 [2]牡丹江医学院附属红旗医院

出　　处：《中国医学创新》2018年第24期19-22,共4页Medical Innovation of China

基　　金：牡丹江市科学技术计划项目(Z2016s0094);牡丹江医学院科学技术研究项目(zs2015-09)

摘　　要：目的:探讨金福菇多糖对衰老大鼠p16基因表达的影响。方法:实验材料选用清洁级SD大鼠25只,将实验动物随机分成5组,每组5只。分别为正常对照组、衰老模型组、高剂量治疗组、中剂量治疗组、低剂量治疗组,采用皮下注射D-半乳糖方法建立衰老大鼠模型,除正常对照组外,分别给予金福菇多糖溶液灌胃治疗,采用逆转录PCR(reverse transcription-PCR,RT-PCR)检测各组大鼠心脏组织p16 mRNA的表达情况。结果:衰老模型组小鼠p16基因表达明显高于正常对照组,差异有统计学意义(P<0.05),给予金福菇多糖治疗后p16基因相对表达均明显低于衰老模型组小鼠,其中中剂量组最低,三组比较差无统计学意义(P>0.05)。结论:金福菇多糖可有效降低p16 mRNA的表达,从而起到抗衰老的作用,值得进一步研究。To explore the effect of Jin Fu Mushroom Polysaccharide on p16’s gene expression of aging rats.Method:A total of25clean grade SD rats were chosen,the animals were randomly divided into5groups,5rats in each group(the normal control group,aging model group,high-dose treatment group,middle-dose group,small-dose treatment group),using the method of subcutaneous injection of D-galactose aging rat model was established,in addition to the normal control group,another groups were given different doses of Jin Fu Mushroom Polysaccharide treatment,the p16gene expression of rats was detected by reverse transcription PCR(reverse transcription-PCR,RT-PCR).Result:The p16gene expression of aging model group was obviously higher than that of the normal control group,the difference was statistically significant(P<0.05),after giving Jin Fu mushroom polysaccharide,the p16’s gene expression was lower than those of in aging model group,in which the lowest dose group,but there was no significant difference among three groups(P>0.05).Conclusion:Jin Fu Mushroom Polysaccharide can effectively reduce the p16mRNA expression,is likely to be the mechanism of heart in the rat model of aging,is worth of further study.

关 键 词：金福菇多糖 衰老 P16 

分 类 号：R285.5[医药卫生—中药学]