狼毒大戟多糖对小鼠脾淋巴细胞增殖及细胞因子分泌的影响  

Effect of Euphorbia Fischeriana Polysaccharide on Proliferation and Production of Cytokines of Splenic Lymphocytes In Mice

作　　者：韩德军 夏宁 郑双 蒋丽艳[1] HAN Dejun;XIA Ning;ZHENG Shuang(Qiqihar Medical University,Qiqihar 161000,China)

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161000

出　　处：《中国医学创新》2018年第24期26-28,共3页Medical Innovation of China

基　　金：黑龙江省大学生创新创业训练项目(201611230044)

摘　　要：目的:探讨狼毒大戟多糖(EFP-AW1)对小鼠脾淋巴细胞增殖及细胞因子白介素2(IL-2)和干扰素γ(IFN-γ)分泌的影响。方法:制备小鼠脾淋巴细胞,加入EFP-AW1,使终质量浓度分别为0、25、50、100、200 mg/L,再加入Con A,培养60 h后,采用MTT法检测不同质量浓度狼毒大戟多糖EFP-AW1对Con A诱导的小鼠脾淋巴细胞增殖反应的影响;将5种不同浓度的EFP-AW1分别加入小鼠脾淋巴细胞培养体系,再加入Con A,培养48 h后,收集培养上清,ELISA法检测不同质量浓度EFP-AW1对小鼠脾淋巴细胞分泌IL-2和IFN-γ的影响。结果:与空白组比较,50、100、200 mg/L的狼毒大戟多糖能够促进Con A诱导的小鼠脾淋巴细胞增殖反应(P<0.05),并能够提高脾淋巴细胞产生IL-2和IFN-γ的水平(P<0.05)。结论:狼毒大戟多糖具有一定的免疫增强作用。To observe the effect of Euphorbia Fischeriana Polysaccharide(EFP-AW1)onproliferation and production of interleukin2(IL-2),interferonγ(IFN-γ)of splenic lymphocytes in mice.Method:Five concentrations of EFP-AW1(0,25,50,100,200mg/L)with Con A were added to the culture system of mouse splenic lymphocytes for60h,the effect of EFP-AW1at different concentrations on splenic lymphocyte proliferation were detected by MTT method.Five concentrations of EFP-AW1(0,25,50,100,200mg/L)with Con A were added to the culture system of mouse splenic lymphocytes for48h,IL-2and IFN-γproduction from splenic lymphocytes were examined by ELISA.Result:Compared with the blank group,EFPAW150,100and200mg/L promoted Con A induced T lymphocyte proliferation(P<0.05),and elevated IL-2and IFN-γproduction from splenic lymphocytes(P<0.05).Conclusion:EFP-AW1has immunity-enhancing effect.

关 键 词：狼毒大戟 多糖 脾淋巴细胞 白介素2 干扰素γ 

分 类 号：R285.5[医药卫生—中药学]