实时荧光PCR探针法和DNA测序法应用于CYP2C9和VKORC1基因检测的对比研究  

Comparison of Real-time Fluorescent PCR Probe Method and DNA Sequencing Method in the Detection of CYP2C9 and VKORC1 Genes

作　　者：黄振勇[1] 龙东娣 毛振敏[1] 肖诚胤[1] 陈素敏 陈清群 蔡凯[1] 李介华[1] HUANG Zhenyong;LONG Dongdi;MAO Zhenmin(The Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan 511518,China)

机构地区：[1]广州医科大学附属第六医院(广东省清远市人民医院),广东清远511518 [2]广东医科大学

出　　处：《中国医学创新》2018年第24期123-127,共5页Medical Innovation of China

基　　金：清远市科技计划项目(2014B020)

摘　　要：目的:比较实时荧光PCR探针法和DNA测序法检测细胞色素氧化酶P450(CYP2C9)和维生素K环氧化物还原酶复合体1(VKORC1)基因多态性的一致性,建立适用于临床推广的华法林基因多态性检测的方法。方法:本研究随机收集清远市人民医院20例就诊患者的全血标本,应用实时荧光PCR探针法和DNA测序法检测其CYP2C9和VKORC1基因的多态性,结果运用Excel软件统计并进行比较分析。结果:两种方法对CYP2C9和VKORC1基因多态性的检测结果一致,且实时荧光PCR探针法比DNA测序法更简易、经济、快捷。结论:与DNA测序法相比,应用实时荧光PCR探针法检测CYP2C9和VKORC1基因的突变具有较高的临床应用价值,比较适用于临床检测。To compare the polymorphism of cytochrome oxidase P450(CYP2C9)and vitamin K epoxide reductase complex1(VKORC1)gene by real-time fluorescent PCR and DNA sequencing.Methods of clinical promotion of warfarin gene polymorphism.Method:The whole blood samples of20patients in Qingyuan People’s Hospital were randomly collected.The polymorphisms of CYP2C9and VKORC1genes were detected by real-time fluorescent PCR and DNA sequencing.The results were analyzed by Excel software and compared.Result:The results of two methods were consistent with the detection of CYP2C9and VKORC1gene polymorphism,and the real-time fluorescent PCR method was simpler,more economical and faster than DNA sequencing method.Conclusion:Compared with DNA sequencing method,real-time fluorescent PCR probe method detection of CYP2C9and VKORC1gene mutations has a high clinical value,is more suitable for clinical testing

关 键 词：实时荧光PCR探针法 DNA测序法 CYP2C9 VKORC1 

分 类 号：R440[医药卫生—诊断学]