NODs在人工髋关节无菌性松动假体周围组织中的表达及相关研究  

Expression and related research of NODs in periprosthetic tissue of aseptic hip joint loosening

作　　者：邝满源 李仕勋 谭健韶[2] 李长川[1] 傅光涛 邱俊雄 彭芃 丁悦[1] KUANG Manyuan;LI Shixun;TAN Jianshao;LI Changchuan;FU Guangtao;QIU Junxiong;PENG Peng;DING Yue(Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China;First People’s Hospital of Foshan,Foshan,Guangdong 528200,China)

机构地区：[1]中山大学孙逸仙纪念医院,广州510120 [2]佛山市第一人民医院,广东佛山528200

出　　处：《中国科技论文》2018年第12期1329-1335,共7页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20130171110076);国家自然科学基金资助项目(81472100;81672186);中山大学重大项目和前沿新兴交叉学科培育资助计划项目(17ykjc16);佛山市"十三五"医学重点专科和特色专科建设项目(FSZDZK135002)

摘　　要：为研究核苷酸结合寡聚化结构域蛋白(nucleotide-binding oligomerization domain proteins,NODs)在磨损颗粒激活巨噬细胞引起的人工关节假体无菌松动中的作用,收集髋关节周围组织共32例,其中髋关节假体无菌性松动组(A组)7例,股骨颈骨折组(B组)8例、股骨头缺血性坏死组(C组)13例、化脓性感染组(D组)4例。采用免疫组织化学染色法检测对比各组织巨噬细胞中NOD1、NOD2的表达情况;并在体外用钛颗粒刺激小鼠RAW264.7巨噬细胞检测其NOD1、NOD2及相关炎症因子的表达。结果表明:免疫组化提示,A组巨噬细胞中NOD1和NOD2的表达水平显著高于其他各组(P<0.05)。体外钛颗粒刺激巨噬细胞后,NOD2、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的mRNA表达在1h达到高峰,明显高于对照组;而NOD1的mRNA表达无显著差异(P>0.05)。但钛颗粒刺激巨噬细胞24h后NOD1、NOD2和TNF-α蛋白表达水平增加。NOD1和NOD2可能参与了磨损颗粒激活巨噬细胞诱导人工关节无菌性松动的过程。To investigate the role of nucleotide-binding oligomerization domain proteins(NODs)in aseptic joint loosening mediated by wear particles,totally 32 cases of tissues fromhip were collected,including 7 cases of periprosthetic tissues from aseptic joint loosening(group A),8 cases from femoral neck fracture(group B),13 cases from femoral head ischemic necrosis(group C),and 4 cases from purulent infection(group D).Immunohistochemical staining was used to detect the expression of NOD1 and NOD2 in macrophages of various tissues.In vitro,titanium particles stimulated mouse RAW264.7 macrophages were used for the detection of NOD1,NOD2 and related inflammatory cytokines expression.The results of immunohistochemistry show that the expression of NOD1 and NOD2 in macrophages of group A is significantly higher than those in other groups(P<0.05).The mRNA expression of NOD2 and tumor necrosis factor-α(TNF-α)in macrophages stimulated by titanium particles in vitro reaches a peak at 1 h,which is significantly higher than that of the control group.However,NOD1 mRNA expression has no significant difference(P>0.05).But the expression of NOD1,NOD2 and TNF-αin the macrophages stimulated by titanium particles increases after 24 h.NOD1 and NOD2 may be involved in the process of aseptic joint loosening mediated by macrophages which are activated by wear debris.

关 键 词：关节外科 人工关节 无菌性松动 核苷酸结合寡聚化结构域蛋白 磨损颗粒 

分 类 号：R687.4[医药卫生—骨科学]