TiO_2纳米材料对前成骨细胞MC3T3-E1增殖分化及免疫毒性影响  

Effect of TiO_2 nanoparticles on MC3T3-E1 cells proliferation,differentiation and its immunotoxicity

作　　者：胡云婧 张小雷 张潇 王江雪[1,2,3] HU Yunjing;ZHANG Xiaolei;ZHANG Xiao;WANG Jiangxue(School of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China;Key Laboratory of Biomechanics and Mechanobiology,Ministry of Education,Beihang University,Beijing 100191,China;Beijing Advanced Innovation Center for Biomedical Engineering,Beihang University,Beijing 100191,China)

机构地区：[1]北京航空航天大学生物与医学工程学院,北京100191 [2]北京航空航天大学生物力学与力生物学教育部重点实验室,北京100191 [3]北京航空航天大学生物医学工程高精尖创新中心,北京100191

出　　处：《中国科技论文》2018年第12期1336-1340,共5页China Sciencepaper

摘　　要：研究不同晶型、不同浓度纳米TiO_2对成骨细胞MC3T3-E1的增殖和分化能力及免疫毒性的影响。采用CCK-8法检测发现,锐钛矿和金红石在不同浓度下(20、50、100μg/mL)均抑制了细胞的增殖能力。相比于金红石材料,锐钛矿材料对细胞增殖有一定的抑制效果,但是不存在显著性差异。共培养7d和14d时,锐钛矿和金红石均导致细胞碱性磷酸酶(alkaline phosphatase,ALP)含量显著下降。酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法测定细胞培养上清液中白介素1(interleukin-1,IL-1)、白介素6(interleukin-6,IL-6)水平,发现纳米TiO_2刺激细胞分泌IL-1,同时大量分泌IL-6。并且,在培养24h和48h时,20μg/mL的锐钛矿相比于金红石纳米TiO_2,引起细胞分泌IL-6的水平更高。因此,纳米TiO_2能够影响MC3T3-E1细胞的增殖、分化能力,并引起免疫毒性。The effect of TiO 2 nanoparticles with different physical characters on MC3T3-E1 cellsproliferation,differentiation and immunotoxity has been investigated.The CCK-8 method shows that the anatase and rutile nano-TiO 2 inhibit cells prolifertaion at the concentration of 20,50,100μg/mL.The anatase nano-TiO 2 shows more inhibitory effect on cells viability than rutile,but there is not statistically different.Coculturing with MC3T3-E1 for 7 d and 14 d,the anatese and rutile both decrease the alkaline phosphatase(ALP)synthesis.The enzyme-linked immunosorbent assay(ELISA)is used to detect the interleukin-1(IL-1)and interleukin-6(IL-6)levels in the cell cultures.By culturing by anatase and rutile at different concentration,MC3T3-E1 secretes IL-1,at the same time largely secretes IL-6.In addition,at the concentration of 20μg/mL,the anatase stimulates cells to secrete more IL-6 than rutile.Therefore,TiO 2 nanoparticles have an influence on the proliferation and differantiation of MC3T3-E1 cells and cause the immunotoxicity.

关 键 词：生物医学工程 TIO2纳米颗粒 MC3T3-E1细胞 增殖 分化 免疫毒性 

分 类 号：R318[医药卫生—生物医学工程]