山东地区10株H9N2亚型禽流感病毒HA和NA基因的遗传演化分析  

PHYLOGENETIC ANALYSIS OF HA AND NA GENES OF TEN STRAINS OF H9N2 SUBTYPE AVIAN INFLUENZA VIRUS ISOLATED FROM SHANDONG PROVINCE

作　　者：王友令[1] 袁小远[1] 孟凯[1] 张玉霞[1] 徐怀英[1] 李莉[1] 亓丽红[1] 宋敏训[1] 艾武[1] WANG You-ling;YUAN Xiao-yuan;MENG Kai;ZHANG Yu-xia;XU Huai-ying;LI Li;QI Li-hong;SONG Min-xun;AI Wu(Institute of Poultry Science,Shandong Academy of Agricultural Science,Jinan 250100,china)

机构地区：[1]山东省农业科学院家禽研究所,济南250100

出　　处：《中国动物传染病学报》2018年第4期35-39,共5页Chinese Journal of Animal Infectious Diseases

基　　金：畜禽重大疫病防控与高效安全养殖综合技术研发(2016YFD0501600);山东省2017年度农业重大应用技术创新项目(肉鸡呼吸道疾病的监测和防控技术集成与示范);山东省农业科学院农业科技创新工程(CXGC2016A10)

摘　　要：为了解2016年山东地区H9N2亚型禽流感病毒的变异情况,选取分离鉴定的10株H9N2亚型禽流感病毒分离毒株,分别对其HA和NA基因进行序列测定和分子特性分析,并应用Mega 5.0软件绘制相应的基因氨基酸进化树,进行遗传进化分析。结果表明:10株H9N2分离毒的HA裂解位点氨基酸均为RSSR/GLF,具有典型低致病性AIV HA基因的特征;其第226位氨基酸均为亮氨酸(L),因此具有结合人流感受体的分子特性。同时这10株病毒的NA基因颈部均有9个核苷酸的缺失,且NA的潜在糖基化位点均不超过8个。进化树分析表明,2016年山东地区H9N2流行毒株HA和NA基因均属A/Chicken/Beijing/1/1994亚群,并且同属于近几年在中国鸡群中流行的G57分支。To evaluate the genetic variations of H9N2 Avian influenza virus(AIV)circulating in 2016 in Shandong province,HA and NA genes of 10 isolates of H9N2 AIV obtained in 2016 were sequenced for phylogenetic analysis using Mega 5.0 software.The results showed that the cleavage sites of their HA genes were all RSSR/GLF,showing the typical gene character of LPAIVs.The HA possessed leucine at 226th amino acid position,indicating a human influenza receptor binding characteristic.The NA genes of these 10 isolates had a 9 bp deletion and their potential glycosylation sites were not more than 8.Phylogenetic analysis of HA and NA genes of these H9N2 AIV isolates indicated that all of them belonged to the A/Chicken/Beijing/1/1994 subgroup and G57 lineage widely circulating among Chinese chicken populations in recent years.

关 键 词：禽流感病毒 H9N2亚型 HA基因 NA基因 遗传演化分析 

分 类 号：S852.659.5[农业科学—基础兽医学]