吸烟者外周血单个核细胞差异表达miRNA与mRNA调控网络  

The regulatory network of dysregulated microRNAs and mRNAs in peripheral mononuclear cells of cigarette smokers

作　　者：党晓敏[1] 曲晓艳 尚东[1] 杨岚[1] 王维嘉 李莹[2] 李飞燕[1] 常颖[2] DANG Xiao-min;QU Xiao-yan;SHANG Dong;YANG Lan;WANG Wei-jia;LI Ying;LI Fei-yan;CHANG Ying(Department of Respiration Critical Medicine,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Center for Translational Medicine,the Key Laboratory of Biomedical Information Engineering of Ministry ofEducation,School of Life Science and Technology and Frontier Institute of Science and Technology,Xi'an Jiaotong University,Xi'an 710054,China)

机构地区：[1]西安交通大学第一附属医院呼吸与危重症医学科,陕西西安710061 [2]西安交通大学前沿科学与技术研究院,生命科学与技术学院,转化医学研究中心,陕西西安710054

出　　处：《西安交通大学学报（医学版）》2018年第5期709-714,751,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.31501044),陕西省国际科技合作与交流计划(No.2016KW-026,No.2017KW-044)。

摘　　要：目的探讨吸烟者外周血单个核细胞(PBMC)差异表达的miRNA与mRNA调控网络及相关信号通路。方法收集20名健康非吸烟者与17名健康吸烟者的外周静脉血,分离PBMC后提取总RNA,分别进行miRNA和mRNA表达谱芯片检测。在差异表达的miRNA和mRNA范围内通过数据库预测miRNA对mRNA的调控关系,并进行信号通路富集分析。结果与健康非吸烟者相比,吸烟者PBMC有miRNA与mRNA的差异表达,其差异表达基因的GO富集分析表明,血小板来源生长因子信号通路、胆囊收缩素受体信号通路、T细胞活化和表皮生长因子受体信号通路是主要的信号通路。结论吸烟者PBMC中差异表达的miRNA与mRNA调控网络可能与某些疾病的潜在危险因素相关。Objective To explore the regulatory network of dysregulated microRNAs and mRNAs and related signaling pathways in peripheral mononuclear cells(PBMCs)of cigarette smokers.Methods The peripheral venous blood was taken from 20 healthy non-smokers and 17 healthy smokers.The miRNA and mRNA expression profile of PBMCs was examined by miRNA and mRNA array.The regulatory relationship between dysregulated miRNAs and mRNAs was predicted and the signaling pathways were enriched.Results Compared with the healthy non-smokers,the smokers had dysregulated miRNAs and mRNAs.The GO enrichment analysis indicated that PDGF signaling pathway,CCKR signaling map,T cell activation and EGF receptor signaling pathway were enriched in the smokers.Conclusion The regulatory network of dysregulated miRNAs and mRNAs in PBMCs of smokers may be related to some potential risk factors for certain diseases.

关 键 词：吸烟 MIRNA 基因芯片 信号通路 

分 类 号：R393[医药卫生—基础医学]