不同抗凝剂对黄鳝外周血抗凝效果的比较研究  

Comparative study of on anticoagulant effects of different anticoagulants on peripheral blood of Monopterus albus

作　　者：陈璇[1] 陈志永[1] 徐敏[1] 范玉顶[2] 刘泽军 夏中曦 刘彧 隗黎丽 钟其旺[1] CHEN Xuan;CHEN Zhi-yong;XU Min;FAN Yu-ding;LIU Ze-jun;XIA Zhong-xi;LIU Yu;WEI Li-li;ZHONG Qi-wang(Jiangxi Agricultural University,College of Biological Science and Engineering,Nanchang 330045,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China)

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045 [2]中国水产科学研究院长江水产研究所,武汉430223

出　　处：《淡水渔业》2018年第5期35-40,共6页Freshwater Fisheries

基　　金：国家自然科学基金项目(31160530;31360634);江西省青年科学基金项目(20114BAB214003);江西省科技支撑项目(20122BBF60074);江西省教育厅科技项目(GJJ13289);大学生创新计划项目(201610410020)

摘　　要：为探讨黄鳝(Monopterus albus)血液适宜抗凝剂种类和最佳使用浓度,实验比较了6种常用抗凝剂对黄鳝外周血的抗凝效果及DNA质量的影响。结果显示:ACD、EDTA、肝素、草酸钾、草酸盐和柠檬酸钠的最佳抗凝终浓度分别为0.10×、0.50‰、0.016‰、0.63‰、0.025×和8.00‰,以上浓度能有效抑制凝血,且血细胞死亡率最低。最佳抗凝浓度下的短暂处理对血细胞DNA的质量无明显影响,但随着处理时间的延长,抽提获得的DNA含量均出现了不同程度的下降,含有ACD和EDTA的血液中DNA的含量下降最多,其次是草酸盐、草酸钾和柠檬酸钠,而加入了肝素的血液中DNA的稳定性最高,其含量无显著下降。The anticoagulant effects of six commonly used anticoagulants on the peripheral blood of Monopterus albus were compared.The suitable kind of anticoagulant and its optimum concentration were discussed.The results showed that the optimum final anticoagulation concentrations of ACD,EDTA,heparin,potassium oxalate,oxalate and sodium citrate were 0.10×,0.50‰,0.016‰,0.63‰,0.025×and 8.00‰,respectively,which could effectively inhibit blood clotting and resulted in lowest mortalities of peripheral blood cell.The transient treatments with the optimum concentrations of anticoagulants would not influence the quality of DNA extracted from blood cell.However,the total amounts of extracted DNA declined in different degrees with the extension of processing time,the total amounts of extracted DNA declined in different degrees.The total DNA from ACD and EDTA led to the highest declination in total DNA,followed by oxalate,potassium oxalate and sodium citrate,whereas heparin-added blood had the highest DNA stability with no significant decrease in DNA content.

关 键 词：黄鳝(Monopterus albus) 外周血 抗凝剂 

分 类 号：S917.4[农业科学—水产科学]