罗氏沼虾病原阴沟肠杆菌药物敏感性测定与分析  

Sensitivity determination and analysis of pathogenic Enterobacter cloacae isolated from Macrobrachium rosenbergii

作　　者：李席席 皮大明 高晓建 陈楠[1] 刘晓丹 张莹莹 付立霞[1] 叶金明[2] 张晓君[1] ZHNAG Yue LI Xi-xi;PI Da-ming;GAO Xiao-jian;CHEN Nan;LIU Xiao-dan;ZHANG Ying-ying;FU Li-xia;YE Jin-ming;ZHANG Xiao-jun(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Yangzhou Aquatic Production Technical Station,Yangzhou 225101,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]扬州市水产生产技术指导站,江苏扬州225101 [3]College of Animal Science and Technology,Yangzhou University

出　　处：《淡水渔业》2018年第5期48-53,共6页Freshwater Fisheries

基　　金：江苏省渔业科技重大项目(D2017-3)。

摘　　要：采用纸片扩散法(K-B法)测试了2017年春季分离自江苏省高邮市罗氏沼虾(Macrobrachium rosenbergii)育苗场的5株阴沟肠杆菌(Enterobacter cloacae)对35种抗菌类药物的敏感性,并采用试管稀释法测定了6种抗菌药物对分离菌株WJ1的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),结果显示:恩诺沙星对阴沟肠杆菌的杀菌效果最好,最小杀菌浓度为4 mg/L;其次是诺氟沙星,最小杀菌浓度为7 mg/L;盐酸土霉素和氟苯尼考的最小杀菌浓度分别为8 mg/L和10 mg/L;红霉素和头孢曲松对阴沟肠杆菌杀菌效果最差。Five strains of Enterobacter cloacae were isolated from Macrobrachium rosenbergii cultured in some hatcheries located in Gaoyou county,Jiangsu province in spring of 2017.The susceptibility of the isolates to 35 antibacterial drugs were tested by antimicrobial susceptibility by Kirby-Bauer disk diffusion method.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of six antibacterials against Enterobacter cloacae were determined by in vitro dilution method.The results showed that enrofloxacin had the best bactericidal activity against E.cloacae.The minimum bactericidal concentration was 4 mg/L,followed by norfloxacin,which the minimum bactericidal concentration was 6 mg/L.The minimum bactericidal concentrations of oxytetracycline hydrochloride and florfenicol were 8 mg/L and 10 mg/L,respectively.Erythromycin and ceftriaxone had the worst effect on pathogenic E.cloacae.

关 键 词：罗氏沼虾(Macrobrachium rosenbergii) 阴沟肠杆菌(Enterobacter cloacae) 药物敏感性 

分 类 号：S941.42[农业科学—水产养殖]