表达甲酰辅酶A转移酶的大肠杆菌对大鼠尿草酸浓度的影响  

Probiotic administration of Escherichia coli Nissle 1917 with FCoAT expression reduces urinary oxalate in rat model

作　　者：赖德辉[1,2] 李名钊[3] 盛明[1,2] 李逊[1,2] 何永忠[1,2] 李天[1,2] 徐桂彬[1,2] 杨炜青[1,2] Lai Dehui;Li Mingzhao;Li Xun;HE Yongzhong;Li Tian;Xu Guibin;Yang Weiqing(Department of Urology,the Fifth Affiliated Hospital,Guangzhou Medical University,Guangzhou 510700,China;Minimally Invasive Technique and Product Translational Center,Guangzhou Medical University,Guangzhou 510700,China;Department of Urology,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]广州医科大学附属第五医院泌尿外科,510700 [2]广州医科大学附属第五医院微创新技术和产品转化中心,510700 [3]中山大学附属第三医院泌尿外科,广州510630

出　　处：《中华腔镜泌尿外科杂志（电子版）》2018年第5期352-355,共4页Chinese Journal of Endourology(Electronic Edition)

基　　金：广州医科大学博士启动项目(2014C40)

摘　　要：目的探讨喂饲表达甲酰辅酶A转移酶(FCoAT)的大肠杆菌Nissle1917(EcN-Frc)对大鼠尿草酸浓度的影响。方法将30只雄性SD大鼠随机分为六组(编号A-F),每组5只。除A组外,其余五组在食物中每克添加1%草酸。B组每次喂食前30 min,喂食1 ml EcN-Frc培养液,C、D、E、F组喂食1 ml EcN-Frc悬液(活细菌数103)。C、D、E、F组喂饲细菌的方式分别为每日1次,每3 d一次,每周一次和观察期内首日喂养一次。实验周期为2周,分别于喂养当日,喂养后3、6、9、14 d分别记录大鼠健康情况和24 h尿草酸排泄量。结果口服ECN-Frc能有效减低高草酸饮食大鼠的24 h尿草酸排泄量。含103活菌数的EcN-Frc在喂养后3 d即可发挥作用。1次喂服103个活菌数的ECN-Frc,2周后仍能有效降低大鼠尿中草酸浓度。所有大鼠观察期内均体健,未见明显异常。结论低剂量口服Ec N-Frc能有效降低高草酸饮食大鼠的24 h尿草酸排泄量,且安全,耐受性好。Objective To study of using E.coli Nissle1917 which can express FCoAT(EcNFrc)as probiotic treatment to reduce urine oxalate in a rat model.Methods Thirty male SD rats were randomly divided into 6 groups(A to F).1%oxalate was added to the diet for rats in group B,C,D,E,F;while rats in group A received regular diet.Furthermore,30 minutes before each feeding,each rats in group B received 1 ml suspension,while those in group C,D,E,F was given 1 ml EcN-Frc suspension(103 viable bacteria).Rats in group C,D,E,F were feeding bacteria every day,once every three days,once a week,respectively.24-hour urine specimens were obtained on days 0,3,6,9,14 by placing the rats in metabolic cages.Results Hyperoxaluria rats due to high dietary oxalate were treated by EcN-Frc.It can effectively reduce the high urinary oxalate level in rat model since day 3 after supplementation.The effect of the decrease proved directly proportional to the dose of EcN-Frc.But,one dose of EcN-Frc(103 viable bacteria)can still effectively reduce urinary oxalate in a 2 weeks period.It showed that EcN-Frc had the ability to stay stable in rat’s intestine and grew well.In addition,during 2 weeks period,all rats kept health with no signs of toxicity.Conclusions Probiotic administration of low dose EcN-Frc can effectively reduce the high urinary oxalate in hyperoxaluria rats.EcN-Frc can stay stable in rat’s intestine and play a role in degrading oxalate.This approach appears to be safe and good tolerance.

关 键 词：酶 大肠杆菌 草酸 治疗 大鼠 随机 

分 类 号：R691.4[医药卫生—泌尿科学]