哈茨木霉对紫羊茅和草地早熟禾的促生及抗性诱导作用  

Growth promotion and resistance induction effect of Trichoderma harzianum on Festuca rubra and Poa pratensis

作　　者：谢琳淼 常春丽 姚志红[2] 王洪瑞 赵冬雪 张荣沭[2] 敖红[1] XIE Linmiao;CHANG Chunli;YAO Zhihong;WANG Hongrui;ZHAO Dongxue;ZHANG Rongshu;AO Hong(College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China;College of Landscape Architecture,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北林业大学园林学院,黑龙江哈尔滨150040

出　　处：《草业科学》2018年第9期2079-2086,共8页Pratacultural Science

基　　金：国家自然科学基金(31370642)

摘　　要：为研究哈茨木霉(Trichoderma harzianum)对紫羊茅(Festuca rubra)和草地早熟禾(Poa pratensis)生长的影响和抗性的诱导作用,以不同浓度的哈茨木霉提取液对两种草进行种子萌发及盆栽试验。结果表明,哈茨木霉可以提高两种草种子的发芽势、发芽率、发芽指数和活力指数,其中以1×10~4cfu·mL^(-1)哈茨木霉提取液的提高效果最为显著(P<0.05)。此外哈茨木霉处理后两种草幼苗的胚根、胚芽长度显著高于对照(P<0.05)。土壤中加入1×10~7cfu·mL^(-1)哈茨木霉可以增加两种草的株高、相对含水量及生物量,并可以提高叶片可溶性蛋白、可溶性糖、脯氨酸含量及超氧化物歧化酶和过氧化物酶的活性。因此,1×10~4 cfu·mL^(-1)的哈茨木霉可以促进紫羊茅和草地早熟禾种子的萌发和幼苗生长;1×10~7cfu·mL^(-1)哈茨木霉对两种草的营养生长有明显的促生效应,并可以诱导两种草抗性的产生。In order to study the effect of growth promotion and resistance induction of Trichoderma harzianum on Festuca rubra and Poa pratensis,a seed germination experiment and a pot culture experiment were conducted with different concentrations of T.harzianum extracts.The results showed that the germination potential,germination rate,germination index,and vigor index of the two species of grass were significantly higher than those of the control species(CK),and the effect of 1×10 4 cfu·mL-1 T.harzianum,in particular,were significant(P<0.05).The radicle length and plumule length of the seedlings were significantly longer than those of the control(P<0.05).The results of the pot experiment showed that 1×10 7 cfu·mL-1 T.harzianum extract could increase the biomass,plant height,and relative water content of F.rubra and P.pratensis;the content of proline,soluble protein,and soluble sugar and the activities of superoxide dismutase and peroxidase in leaves of the two species of grass were greater than those of leaves of the CK.In summary,1×10 4 cfu·mL-1 T.harzianum had a better effect on seed germination and seedling growth of the two species of grass,while 1×10 7 cfu·mL-1 T.harzianum could promote growth and induce resistance in the vegetative growth phase of P.pratensis and F.rubra.

关 键 词：哈茨木霉 草地早熟禾 紫羊茅 种子萌发 促生 抗性诱导 

分 类 号：S543.903.4[农业科学—作物学] Q945.79[生物学—植物学]