应用Rusitec-S系统研究甘露寡糖对绵羊体外瘤胃发酵的影响  

Effect of mannan oligosaccharides on ruminal fermentation of sheep in an in vitro Rusitec-S system

作　　者：张然 郑琛[1] 韩海珠[1] 雒瑞瑞 李彦珍 朱伟 ZHANG Ran;ZHENG Chen;HAN Haizhu;LUO Ruirui;LI Yanzhen;ZHU Wei(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《草业科学》2018年第9期2262-2269,共8页Pratacultural Science

基　　金：国家自然科学基金(31560646)

摘　　要：本研究应用Rusitec-S型人工瘤胃模拟装置,采用单因素完全随机设计,在基础饲粮中分别添加0、0.8%、1.6%和2.4%的甘露寡糖(mannan oligosaccharides,MOS),测定总产气量、甲烷产量、养分降解率及培养液pH、总氮、氨氮、尿素氮、蛋白氮和挥发性脂肪酸浓度以及比例的变化,分析饲粮中添加甘露寡糖对绵羊体外瘤胃发酵的影响。试验共两期,每期9d(预试期7d,采样期2d),每个处理4个重复。结果表明,添加甘露寡糖对体外培养液pH、总氮、氨氮、尿素氮、蛋白氮和总挥发性脂肪酸浓度、各挥发性脂肪酸比例、养分降解率及甲烷产量和总产气量均没有产生显著影响(P>0.05)。由此可知,饲粮中添加甘露寡糖在人工瘤胃试验中不会影响瘤胃发酵模式。In this study,a single-factor experimental design was used to explore the effect of mannan oligosaccharides(MOS)on sheep rumen fermentation using a Rusitec-S artificial ruminal system.The basal diet was designed based on the standard Chinese ram nutrient requirements as the control,and the doses of MOS were 0.8%,1.6%,and 2.4%.The experiment was repeated twice;each stage lasted for 9 d,including 7 d acclimation and 2 d experimentation,with four replicates in each treatment.The results showed that there were no significant influences on pH,total volatile fatty acid(TVFA)concentration,total nitrogen,ammonia nitrogen,urea nitrogen,protein nitrogen,ratio of acetate/propionate,acetate,propionate,butyrate and other acids of cultivated fluid,methane yield,and gas production by MOS(P>0.05).In conclusion,the in vitro model of ruminal fermentation was not affected by the addition of MOS using the Rusitec-S system.

关 键 词：Rusitec-S 甘露寡糖 瘤胃发酵 绵羊 甲烷产量 养分降解率 挥发性脂肪酸 

分 类 号：S826.5[农业科学—畜牧学]