应用环介导等温扩增技术检测江苏水稻种子携带的水稻恶苗病菌  

Detection of Seed-borne Rice Bakanae Pathogens in Jiangsu Province, China Using Loop-mediated Isothermal Amplification Assays

作　　者：袁咏天 戎振洋 叶文武[1] 张红生[2] 庄义庆 郑小波[1] YUAN Yongtian;RONG Zhenyang;YE Wenwu;ZHANG Hongsheng;ZHUANG Yiqing;ZHENG Xiaobo(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;College of Agronomy,Nanjing Agricultural University,Nanjing 210095,China;Zhenjiang Agric-academy of Sciences,Zhenjiang 212400,China)

机构地区：[1]南京农业大学植物保护学院,南京210095 [2]南京农业大学农学院,南京210095 [3]镇江市农业科学院,江苏镇江212400

出　　处：《中国水稻科学》2018年第5期493-500,共8页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2012AA101501)

摘　　要：【目的】本研究旨在了解江苏省水稻主栽品种种子携带水稻恶苗病原菌的情况。【方法】采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)对江苏65个水稻主栽品种种子携带藤仓镰孢、层出镰孢、拟轮枝镰孢和Fusarium andiyazi等4种水稻恶苗病菌进行检测。【结果】65个品种中有54个品种的种子样本携带恶苗病菌,表明江苏省稻种携带水稻恶苗病菌是一种普遍现象。其中,藤仓镰孢为优势种,检出率高达83.07%,其次是层出镰孢和Fusarium andiyazi,检出率分别为12.30%、6.15%,但供试的65个品种种子均未检测到携带有拟轮枝镰孢。【结论】该技术可用于水稻种子携带恶苗病菌的快速检测。【Objective】The research aims to reveal the situation of seed-borne rice bakanae pathogens in Jiangsu Province,China.【Methods】The seeds of 65 major rice cultivars collected from this area were analyzed by using loop-mediated isothermal amplification(LAMP)assays for specific detection of 4 known rice bakanae pathogen species,including Fusarium fujikuroi,F.proliferatum,F.verticilliodes and F.andiyazi.【Results】There were 54 rice cultivars carrying rice bakanae pathogens,indicating it’s a common phenomenon that rice seeds carrying rice bakanae pathogens in Jiangsu Province.And the most frequent species was F.fujikuroi,representing 83.07%of the total samples tested,followed by F.proliferatum and F.andiyazi,representing 10%and 6%respectively.However,none of the 65 rice cultivars was tested to be carrying F.verticilliodes.【Conclusions】The method is readily available for detecting seed-borne rice bakanae pathogens.

关 键 词：水稻恶苗病 种子带菌检测 环介导等温扩增技术 

分 类 号：S435.111.44[农业科学—农业昆虫与害虫防治] S511.041[农业科学—植物保护]