孕鼠注射海洛因对仔鼠小脑组织和抗氧化物酶活性的影响  

Effect on the cerebellar tissue and antioxidant enzymes activities of newborn mice after injected heroin in pregnant mice

作　　者：俞诗源[1] 何玲慧 朱嘉宁[1] 陈诚[1] 王小勇[1] YU Shi-yuan;HE Ling-hui;ZHU Jia-ning;CHEN Cheng;WANG Xiao-yong(College of Life Science,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2018年第5期63-67,共5页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省自然科学基金资助项目(1107RJZA141);兰州市社会发展项目(2013-3-72;2014-1-105)

摘　　要：为探讨孕鼠注射海洛因后对仔鼠小脑组织与抗氧化物酶活性的影响,对48只孕鼠(第9天开始)每天早晚分别注射浓度为2,4和6g·L-1的海洛因溶液和等量生理盐水(每天2次,每次0.2mL)直到分娩后第15d,称量检测仔鼠体重和小脑重的变化,用比色法检测小脑组织谷胱甘肽(GSH)、丙二醛(MDA)含量及过氧化氢酶(CAT)活性的变化,用光学显微技术观察小脑组织结构的变化.结果表明,与对照组相比,海洛因组仔鼠体重、小脑重及小脑指数均降低,仔鼠小脑组织出现不同程度的病理变化,细胞排列疏松而凌乱,浦肯野细胞形态不规则、核固缩,细胞出现空泡化和凋亡等现象;仔鼠小脑组织CAT活性及GSH含量均降低(P<0.05),MDA含量则升高(P<0.05),表明孕鼠注射海洛因对仔鼠小脑组织结构与抗氧化功能有明显的损伤效应.To investigate the effect of heroin on cerebellum tissue structure and antioxidant enzymes activities of newborn mice,48 pregnant mice are separately injected different concentrations(2,4 and 6 g·L-1)heroin which is content 0.2 mL and equivalent saline until delivery 15 day(twice a day).The changes of weight and cerebellum weight are measured at 5,10 and 15 days after birth and the activity of catalase(CAT).The contents of glutathione(GSH),malondialdehyde(MDA)detected by colorimetric method and the microstructure of cerebellum are observed with optical microscopy.The results show that the body weight,cerebellum weight and cerebellum index of heroin group are reduced than the control group.There are different degrees pathological changes in the cerebellum tissue,the cells are loosely and disorderly arrange.The shape of purkinje cell morphology is irregular.Nuclear pyknosis,vacuolization and apoptosis appears in cell.Catalase(CAT)activity and glutathione(GSH)content in the cerebellum of offspring rats decrease(P<0.05),and malondialdehyde(MDA)content increased(P<0.05).Above results indicate that heroin injection has significantly scathing effect on the structure and antioxidant function of cerebellum in offspring rats.

关 键 词：海洛因 小脑组织 过氧化氢酶(CAT) 谷胱甘肽(GSH) 丙二醛(MDA) 

分 类 号：R285.5[医药卫生—中药学] D919.4[医药卫生—中医学]