玉米南方锈病抗病QTL鉴定和效应分析  

Mapping and effectiveness analysis for resistance genes of southern corn rust in maize

作　　者：艾堂顺 田志强[1] 李会敏 邓策 丁俊强[1] 张学林[1] 刘海富 朱伟岭 李志敏[1] AI Tangshun;TIAN Zhiqiang;LI Huimin;DENG Ce;DING Junqiang;ZHANG Xuelin;LIU Haifu;ZHU Weiling;LI Zhimin(College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China;Henan Province Engineering Research Center for Crop Stress Acclimatization,Henan University,Kaifeng 475001,China;Henan Dingyou Agricultural Science and Technology Co.Ltd.,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南大学植物逆境生物学重点实验室,河南开封475001 [3]河南鼎优农业科技有限公司,河南郑州450046

出　　处：《河南农业大学学报》2018年第4期514-518,共5页Journal of Henan Agricultural University

基　　金：国家自然科学基金委青年科学基金项目(31501326)

摘　　要：以玉米抗病自交系P 178、感病自交系G 41构建的BC2F5群体为材料,通过遗传连锁图谱构建和表型鉴定,对玉米南方锈病抗性进行了QTL定位。结果表明,在玉米第2,5,6和10染色体上共鉴定出5个抗病位点,可以解释6.88%到45.31%的表型遗传变异。其中,位于第10染色体短臂上的位点q SCR10.01具有最大的效应值。进一步对qSCR10.01进行精细定位的结果表明,抗病基因位于标记UMC1380和C(10)3595071之间,标记间的物理距离为1.34 Mb。This study was to use quantitative trait loci(QTL)technique to map the resistance genes of southern corn rust(SCR)through genetic linkage map construction and phenotypic identification.The model material BC2F5 population was developed from the cross P178×G41,in which the line P178 was resistant to SCR and G41 was susceptible to SCR,respectively.Combined with the construction of genetic map with 349 KASP markers,quantitative trait loci(QTL)for SCR score was analyzed.Five QTL were identified on chromosomes 2,5,6 and 10,respectively,representing 6.88%~45.31%phenotypic variation.Of the five QTL identified,the one on chromosome 10(qSCR10.01)has biggest effect on,which can explain 45.31%phenotypic variation.The results also showed that,by further narrowing down of major QTL qSCR10.01,the QTL region was limited in between of flanking markers UMC1380 and C(10)3595071,which was 1.34 Mb in size based on the B73 reference genome.

关 键 词：玉米 南方锈病 分子标记 主效QTL 精细定位 

分 类 号：S513[农业科学—作物学]