阳荷不同极性部位体外抑菌活性研究  

Study on Antibacterial Activity of Different Polar Extracts from Zingiber striolatum Diels in vitro

作　　者：蔡依 郭百臻 徐津林 李肖 段金辉 阮金兰[1,2] CAI Yi;GUO Bai-zhen;XU Jin-lin;LI Xiao;DUAN Jin-hui;RUAN Jin-lan(School of Life Science,Wuchang University of Technology,Wuhan 430223,Hubei,China;School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430073,Hubei,China)

机构地区：[1]武昌理工学院生命科学学院,湖北武汉430223 [2]武汉工程大学环境生态与生物工程学院,湖北武汉430073

出　　处：《食品研究与开发》2018年第19期36-39,103,共5页Food Research and Development

基　　金：湖北省协同创新中心项目(生物多肽糖尿病药物)(鄂教科涵[2012]56号);湖北省生物多肽糖尿病药物工程技术研究中心项目(鄂科技通[2014]72号)

摘　　要：为研究阳荷的体外抑菌活性,采用70%乙醇回流提取阳荷粉末,依次用石油醚、乙酸乙酯、正丁醇萃取乙醇提取物,得到乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物;用滤纸片对乙醇提取物及不同极性萃取物进行抑菌活性测定,有抑菌活性的部位进一步测定其最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimum bactericidal concentration,MBC)。试验结果表明阳荷乙醇提取物、石油醚萃取物、乙酸乙酯萃取物和正丁醇萃取物,MIC值在3.125 mg/mL^12.5 mg/mL之间,MBC值为25 mg/mL;阳荷乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物对枯草芽孢杆菌有较强的抑菌效果,MIC值在3.125 mg/mL^12.5 mg/mL之间,MBC值为25 mg/mL;阳荷乙醇提取物和石油醚萃取物对大肠杆菌、肺炎克雷伯菌、粪肠球菌、铜绿假单胞菌和鲍曼不动杆菌均有抑菌效果;阳荷乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水萃取物对苏云金芽孢杆菌没有抑菌作用。阳荷对革兰氏阳性菌和革兰氏阴性菌具有广谱抑菌活性。In order to study the antibacterial activity of Z.striolatum Diels in vitro,the Z.striolatum Diels powder were refluxed by the 70%ethanol,in turn with petroleum ether,ethyl acetate,n-butanol extracted ethanol extract,in order to obtain ethanol extract,petroleum ether extract,ethyl acetate extract,n-butanol extract and water extract;the antibacterial activity of different polar extract were measured with a filter paper,and the minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)were further measured which had antibacterial activity.The experimental results showed that the ethanol extracts、petroleum ether extract,ethyl acetate extract and n-butanol extract of Z.striolatum Diels had stong antimicrobial activities on Staphylococcus aureus,MIC value range from 3.125 mg/mL to 12.5 mg/mL,MBC value 25 mg/mL;the ethanol extract,petroleum ether extract,ethyl acetate extract n-butanol extract and water extract of Z.striolatum Diels had strong antibacterial activity on Bacillus subtilis,MIC value range from 3.125 mg/mL to 12.5 mg/mL and MBC value 25 mg/mL;ethanol extract and petroleum ether extract of Z.striolatum Diels had antibacterial effects on Escherichia coli,Klebsiella pneumoniae,Enterococcus faecalis,Pseudomonas aeruginosa and Acinetobacter baumannii;ethanol extract,petroleum ether extract,ethyl acetate extract,n-butanol extract and water extract had no antibacterial activity on Bacillus thuringiensis.Z.striolatum Diels had broad spectrum antibacterial activity against gram-positive and gram-negative bacteria.

关 键 词：阳荷 乙醇提取物 抑菌活性 极性部位 最低抑菌浓度 最低杀菌浓度 

分 类 号：R285[医药卫生—中药学]