淫羊藿苷干预自然衰老模型大鼠大脑皮质趋化因子Fractalkine及其受体CX3CR1的表达  

Icariin affects the expression of fractalkine and its receptor CX3CR1 in cerebral cortex of natural aging rat models

作　　者：张欣 康增军[2] 薛娟 张顺[3] 刘小青[4] 刘未华 Zhang Xin;Kang Zeng-jun;Xue Juan;Zhang Shun;Liu Xiao-qing;Liu Wei-hua(Medical College of Hebei University,Baoding 071000,Hebei Province,China;Department of Neurology,Affiliated Hospital of Hebei University,Baoding 071000,Hebei Province,China;Department of Pathology,Affiliated Hospital of Hebei University,Baoding 071000,Hebei Province,China;Department of Neurosurgery,Baoding No.1 Central Hospital,Baoding 071000,Hebei Province,China)

机构地区：[1]河北大学医学院,河北省保定市071000 [2]河北大学附属医院神经内科,河北省保定市071000 [3]河北大学附属医院病理科,河北省保定市071000 [4]保定市第一中心医院神经外科,河北省保定市071000

出　　处：《中国组织工程研究》2018年第32期5203-5208,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省科技计划项目(132777188);深圳市科技计划项目(JCYJ201504011163247219)~~

摘　　要：背景:趋化因子Fractalkine(FKN)不仅涉及到趋化、黏附和炎症反应,在脑组织中也参与神经细胞的增殖和凋亡。目的:观察淫羊藿苷对自然衰老大鼠大脑皮质趋化因子Fractalkine及其受体CX3CR1的影响。方法:选用3月龄雄性SD大鼠80只,随机分为4组:青年组、自然衰老组、淫羊藿苷低剂量组、淫羊藿苷高剂量组。淫羊藿苷组从16月龄开始分别灌胃淫羊藿苷30,60 mg/kg,连续60 d,青年组和自然衰老组灌胃等量生理盐水60d至取材前。采用苏木精-伊红染色法观察大鼠脑组织形态学变化;β-半乳糖苷酶染色测定脑组织衰老情况;免疫组织化学检测皮质趋化因子Fractalkine和CX3CR1的表达;酶联免疫吸附法检测脑组织中白细胞介素6和肿瘤坏死因子α水平;免疫印迹检测脑组织Caspase-3蛋白表达,比色法检测Caspase-3活性。结果与结论:(1)自然衰老组趋化因子Fractalkine表达明显低于青年组,CX3CR1表达、Caspase-3表达及活性、SA-β-gal阳性颗粒数、炎性指标白细胞介素6、肿瘤坏死因子α明显高于青年组;而淫羊藿苷用药后能逆转上述现象,且淫羊藿苷高剂量组好于淫羊藿苷低剂量组;(2)结果提示淫羊藿苷能够低脑组织中的炎性因子水平,抑制神经细胞凋亡,可能与影响趋化因子Fractalkine及CX3CR1表达有关。BACKGROUND:Chemokine fractalkine not only involves chemotaxis,adhesion,and inflammation,but also participates in the proliferation and apoptosis of nerve cells in brain tissue.OBJECTIVE:To observe the effect of icariin on the expression of fractalkine and its receptor CX3CR1 in cerebral cortex of natural aging rats.METHODS:Eighty 3-month-old male Sprague-Dawley rats were randomly divided into four groups:young group,natural aging group,lowand high-dose icariin groups.Rats in the young and natural aging groups were intragastrically administered equal amount of normal saline for 60 days.Starting from 16 months old,rats in the drug groups were given 30 and 60 mg/kg icraiin for 60 days.Pathological changes in brain morphology were observed by hematoxylin-eosin staining.Brain senescence was identified byβ-galactosidase staining.The expression of fractalkine and CX3CR1 was detected by immunohistochemistry.The levels of interleukin 6 and tumor necrotic factorαwere tested by ELISA.The expression and activity of caspase-3 was determined by western blot assay and colorimetry,respectively.RESULTS AND CONCLUSION:The expression level of fractalkine in the natural aging group was significantly lower than that in the young group.The expression of CX3CR1,the expression and activity of caspase-3,the number of positive particles of SA-β-gal,and the levels of interleukin 6 and tumor necrotic factorαin the natural aging group were significantly higher than those in the young group.After administration of icariin especially at high doses,the above phenomena were reversed.These results suggest that icariin can reduce the levels of inflammatory factors and inhibit apoptosis,which may be via regulating the expression of fractalkine and its receptor CX3CR1.

关 键 词：淫羊藿苷 自然衰老 趋化因子FRACTALKINE CX3CR1 白细胞介素6 肿瘤坏死因子 组织构建 淫羊藿属 衰老 趋化因子类 半胱氨酸天冬氨酸蛋白酶3 组织工程 

分 类 号：R318[医药卫生—生物医学工程]