25份鸢尾属植物基因组DNA C值的流式测定  

Estimation of Genomic C Value of 25 Samples of Iris Plants by Flow Cytometry

作　　者：林峰[1] 肖月娥[2] 周翔宇[3] 唐颖[1] 高步红[1] LIN Feng;XIAO Yue-e;ZHOU Xiang-yu;TANG Ying;GAO Bu-hong(Nanjing Forestry University,Nanjing,Jiangsu Province 210037,China;Shanghai Botanical Garden,Shanghai 200231,China;Shanghai Chenshan Botanical Garden,Shanghai 201602,China)

机构地区：[1]南京林业大学,江苏南京210037 [2]上海植物园,上海200231 [3]上海辰山植物园,上海201602

出　　处：《草地学报》2018年第4期985-990,共6页Acta Agrestia Sinica

基　　金：江苏省林业三新工程项目(LYSX[2015]17);江苏高校优势学科建设工程资助项目(PAPD);江苏省高校协同创新计划(CIPJHE);上海市科学技术委员会科研项目(16391900202)资助

摘　　要：为了了解鸢尾属植物的基因组特点,本试验以25份鸢尾属植物的嫩叶为材料,其中13份以番茄嫩叶为内标、9份以玉米嫩叶为内标,3份采用玉米、番茄嫩叶两种内标,以LB01为解离液,以碘化丙啶(PI)为荧光染料,运用流式细胞仪,检测、收集内标与鸢尾属植物DNA峰形,通过计算获得鸢尾属植物的的DNAC值(指生物体不复制的单倍体细胞核所含DNA含量):从2.62~6.77pg不等。结果表明:25份鸢尾属的基因组DNA的C值可分为15组,组间具有显著差异;3份双内标的方差分析表明,同一份材料采用两种内标进行计算得到的C值没有显著差异。本研究可为鸢尾属植物资源的收集、鉴定、保护和育种等提供理论依据。In order to understand the genomic characteristics of Iris plant,determination of the genome size of Iris was an preliminary task.Fresh leaves of 25 samples of Iris plants were taken as the materials,of which 13 samples were using fresh leaves of tomato as internal reference,9 samples with fresh leaves of corn as internal reference,3 samples were using fresh leaves of tomato and corn as internal reference,with LB01 as dissociation liquid,propidium iodide(PI)as fluorescent dye.By using flow cytometry to detection and collect the internal reference and Iris DNA peak,obtained by calculating the Iris DNA C value(refers to the DNA content of haploid nuclei that are not replicated by organisms)ranging from 2.62~6.77 pg.The results showed that the genomic DNA C value of 25 Iris samples can be divided into 15 groups,with significant differences between the groups;the variance analysis of 3 double internal reference showed that the same material with the calculated C value between two kinds of internal reference value had no significant difference.The study can provide theoretical basis for the collection,identification,protection and breeding of Iris plant.

关 键 词：鸢尾属 基因组大小 流式细胞术 

分 类 号：Q949[生物学—植物学]