Halogranum amylolyticum TNN58全基因组测序及PHBV代谢途径分析  

Genomic Sequencing and Analysis of Genes Related to PHBV of Halogranum amylolyticum TNN58

作　　者：赵有玺[1,2,3] 薛燕芬 马延和[3] 饶志明[1] ZHAO Youxi;XUE Yanfen;MA Yanhe;RAO Zhiming(Key Lab of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;Biochemical Engineering College,Beijing Union University,Beijing 100023,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036 [2]北京联合大学生物化学工程学院,北京100023 [3]中国科学院微生物研究所,北京100101

出　　处：《食品与生物技术学报》2018年第6期572-579,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21276110);国家973计划项目(2012CB725202);江苏高校优势学科建设工程项目;北京市优秀人才培养资助青年骨干个人项目((2014000020124G064));111计划(111-2-06);生物质废弃物资源化利用北京市重点实验室开放课题项目

摘　　要：解淀粉盐颗菌TNN58(Halogranum amylolyticum TNN58)是一株极端嗜盐古菌,它可以利用葡萄糖积累3HV摩尔分数高达20%的PHBV。首次通过鸟枪法对H.amylolyticum TNN58进行了全基因组测序,利用Velvet软件进行组装,得到32个支架序列,整个基因组大小约为4.71Mb,测序深度为218X。通过对基因组数据进行分析,发现该菌株PHA合酶由pha E和pha C两个亚基组成,存在4条3-HV的供应途径,同时发现该菌株不存在乙醛酸循环途径,但存在甲基天冬氨酸循环途径作为乙酸盐的回补利用途径。本研究的结果为揭示H.amylolyticum TNN58的PHBV合成机制和后续的功能基因组学研究的提供了数据。Halogranum amylolyticum TNN58 belong to haloarchaeon.It could produce a type of PHBV containing up to 20 mol%3HV from glucose.This study uses shot-gun sequencing technology to uncover the genome sequence of H.amylolyticum TNN58.Then use Velvet software stitching get 32 scaffolds.The whole genome is about 4.71 Mb,the sequencing depth was 218X.According to the analysis of genome sequence,the PHA sythaseis composed of PhaE and PhaC subunits and there is no acetaldehyde acid cycle in strain.It is interesting to find the methylaspartate cycle is an anaplerotic acetate assimilation pathway.These results will help to reveal the mechanism of PHBV synthesis and provide genome sequence for the research on H.amylolyticum TNN58.

关 键 词：PHBV 基因组 解淀粉盐颗菌 

分 类 号：Q789[生物学—分子生物学]