乌龙江湿地土壤真菌群落结构的PCR-DGGE分析  

Fungal Community in Soil of Wulongjiang Wetlands Analyzed Using PCR-DGGE

作　　者：陈丽华 吕新 刘兰英 黄薇 李玥仁 CHEN Li-hua;Lv Xin;LIU Lan-ying;HUANG Wei;LI Yue-ren(Institute of Agricultural Quality Standards and Testing Technology Research,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所,福建福州350003

出　　处：《福建农业学报》2018年第8期835-841,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2009J01099);福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1025-7);福建省农业科学院青年人才创新基金(2014QB-31)

摘　　要：为了解乌龙江湿地土壤真菌群落结构组成及动态变化,本研究通过稀释平板法对湿地土壤可培养真菌数进行测定,并采用变性梯度凝胶电泳(DGGE)技术分析乌龙江湿地土壤真菌群落结构。平板计数结果表明,不同时间不同采样点可培养真菌数的变化幅度较小,每克干土中可培养真菌数介于0.13×10~4~8.26×10~4 CFU。真菌18SrDNA的PCR-DGGE图谱显示,每个泳道条带的位置、数目和亮度各有不同,说明不同采样时间不同采样点土壤真菌群落结构存在着差异。DGGE图谱条带多样性分析结果表明,2009年3月、2009年11月和2010年1月所采集土壤样品的真菌群落多样性较其他时间更为丰富;从采样点来看,真菌群落丰富度则表现为样品L>样品M>样品R。对DGGE主要条带和差异性条带进行克隆测序后发现,乌龙江湿地土壤主要真菌类群为子囊菌Ascomycota和毛霉菌Mucoromycota。Composition and dynamics of the fungal community in soil of Wulongjiang wetlands were studied.The classical plate count and the denatured gradient gel electrophoresis(DGGE)were applied to determine the cultivable population and diversity of the community.The plate counts of the samples averaged between 0.13×10 4 and 8.26×10 4 CFU per g of dry soil with no significant difference due to the locations or time of collection.On the other hand,the PCR-DGGE patterns of 18S rDNA(V1+V2)fragments exhibited variations on the positions,number and lightness of the bands from different lanes,indicating compositional differences among the fungal populations.The DGGE bands digitally analyzed by Quantity One software and the derived Shannon-Wiener index,evenness and abundance showed greater diversities in the samples obtained in March 2009,September 2009 and January 2010 than other times of a year.They also unveiled the differences in abundance of Sample L>Sample M>Sample R.The dominant fungi in the soil were identified to be Ascomycota and Mucoromycota according to a sequence analysis on the DGGE bands.

关 键 词：乌龙江湿地 土壤真菌 群落结构 PCR-DGGE 多样性分析 

分 类 号：Q938.1[生物学—微生物学]