失神经化骨质疏松动物模型的建立与评价  

Establishment and evaluation of denervated osteoporosis animal model

作　　者：黄昌钊 王锐英[1] 胡译文[2] HUANG Changzhao;WANG Ruiying;HU Yiwen(Department of Limb Trauma Bone Surgery,Affiliated Hospital of Guilin Medical College,Guilin,Guangxi,541001,China;Department of Spinal Bone Surgery,Affiliated Hospital of Guilin Medical College,Guilin,Guangxi,541001,China)

机构地区：[1]桂林医学院附属医院四肢创伤骨外科,广西桂林541001 [2]桂林医学院附属医院脊柱骨病外科,广西桂林541001

出　　处：《重庆医学》2018年第26期3368-3371,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81460198);广西壮族自治区教育厅科研资助项目(201010LX330)

摘　　要：目的通过切断臂丛神经构建废用性骨质疏松动物模型,探讨周围神经损伤对局部骨质影响,为失神经化骨质疏松研究提供实验载体。方法取大白兔左下颌骨经颈向下至胸大肌中部连线切口,镜下分离左全臂丛至神经根孔处剪断,构建臂丛神经根性损伤模型。于术后第4、8、12周3个时间点检测双侧肱骨密度及干重,三点折弯试验检测骨力学性能,HE及Masson染色观察骨组织形态。结果与对照侧相比,各组实验侧骨密度及骨干重进行性减少,各组实验侧的骨力学强度指标明显小于对照侧,差异有统计学意义(P<0.05)。各组实验侧骨小梁数量、小梁间吻合、孔隙大小及破骨细胞数目与对照侧相比差异有统计学意义(P<0.05)。Masson染色表明随失神经化肢体废用时间延长,新生骨胶原数量显著减少(P<0.05)。结论臂丛神经切断术建立失神经化废用骨质疏松动物模型有效可行,骨的质量及结构均随着失神经化时间的延长而发生不同程度病理改变。Objective To establish the disuse osteoporosis animal model by cutting off the brachial plexus and to investigate the effects of peripheral nerve injuries on the local bone substance so as to provide the experimental carriers for the study of denervated osteoporosis.Methods The connection incision from white rabbits left mandible through the neck downward to the middle part of the pectoralis major was taken and the left brachial plexus to the nerve root foramen was microscopically isolated and cut,then the brachial plexus nerve root injury model was constructed.The density and dry weight of humerus were measured at 3 time points of postoperative 4,8,12 weeks,and the mechanical properties of bone were detected by three point bending test.The bone histological morphology was observed by HE and Masson staining.Results Compared with the control side,the bone mineral density(BMD)and bone dry weight of the experimental side in each group were progressively decreased,and the bone mechanical strength indexes of experimental side in each group were significantly smaller than those of the control side,and the differences were statistically significant(P<0.05).The number of bone trabeculas,trabecular anastomosis,pore size and number of osteoclasts of the experimental side in each group were significantly different from those of the control side(P<0.05).The Masson staining showed that the number of new bone collagen was decreased significantly with lengthening of denervation limb disuse time.Conclusion Brachial plexus neurotomy is an effective and feasible method to establish denervated osteoporosis animal model.The quality and structure of bone develop different degrees of pathological changes with the prolongation of denervation time.

关 键 词：失神经化 骨质疏松 力学性能 臂丛神经 动物模型 

分 类 号：R681.7[医药卫生—骨科学]