IL-10基因多态性与中国北方人群糖尿病肾病易感性的关系  

Association between interleukin-10 gene polymorphisms and susceptibility of diabetic nephropathy in northern population of China

作　　者：马东红[1] 刘云[1] 石岩 许清玉[1] 郭明好[1] MA Donghong;LIU Yun;SHI Yan;XU Qingyu;GUO Minghao(First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China)

机构地区：[1]新乡医学院第一附属医院,河南卫辉453100

出　　处：《重庆医学》2018年第26期3390-3393,共4页Chongqing medicine

基　　金：2017年度河南省高等学校重点科研项目(17A320026)

摘　　要：目的探讨白细胞介素(IL)-10-1082G/A、-819T/C和-592A/C的遗传变异性与中国北方人群糖尿病肾病易感性之间的关系。方法收集该院2012年6月到2015年12月确诊为2型糖尿病及糖尿病肾病的患者224例为观察组,选取同期在该院进行常规健康检查的健康人350名作为对照组。通过聚合酶链反应-限制性片段长度多态性对IL-10-1082G/A、-819T/C和-592A/C基因多态性进行分型。结果与对照组相比,观察组患者IL-10-1082G/A中GG、GA和AA的基因频率差异有统计学意义(χ2=7.729,P=0.021)。非条件Logistic回归分析显示IL-10-1082G/A的AA基因型相比于野生型基因显著增加了糖尿病肾病的易感性(OR=2.147,95%CI=1.225~3.762)。此外,与G等位基因相比,A等位基因增加了罹患糖尿病肾病的风险(OR=1.404,95%CI=1.095~1.800)。IL-10-819T/C和-592A/C基因多态性未增加罹患糖尿病肾病的风险。结论 IL-10-1082G/A的基因多态性与糖尿病肾病的发生发展有密切的关系。Objective To study the association between interleukin-10-1082G/A,-819T/C,-592A/C hereditary variability and susceptibility of diabetic nephropathy in northern population of China.Methods A total of 224 cases of type 2 diabetes mellitus(T2DM)and diabetic nephropathy in this hospital from June 2012 to December 2015 were collected as the observation group,and contemporaneous350 individuals undergoing the routine healthy physical examination were selected as the control group.The IL-10-1082G/A,-819T/C and-592A/C polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism.Results Compared to the control group,the difference in GG,GA,and AA gene frequency of IL-10-1082G/A in observation group had statistical significance(χ2=7.729,P=0.021).The non-conditional Logistic regression analysis showed that compared to the wild-type genotype,the AA genotype of IL-10-1082G/A significantly increased the susceptibility of diabetic nephropathy,odds ratio(OR)=2.147,95%confidence interval(CI)=1.225-3.762.Moreover,the A allele increased the risk suffering from diabetic nephropathy compared to the G allele(OR=1.404,95%CI=1.095-1.800).However,the IL-10-819T/C and-592A/C genetic polymorphisms did not increase the risk suffering from diabetic nephropathy.Conclusion The IL-10-1082G/A gene polymorphism has close relationship with the occurrence and development of diabetic nephropathy.

关 键 词：糖尿病肾病 IL-10-1082G/A IL-10-819T/C IL-10-592A/C 多态性 单核苷酸 

分 类 号：R692.6[医药卫生—泌尿科学]