胎盘葡萄糖转运蛋白3在胎儿生长受限胎盘中的表达和意义  

Expression and significance of placenta glucose transporter 3 in fetal growth restriction

作　　者：李金艳[1] 胡荣静 曾敏[2] LI Jinyan;HU Rongjing;ZENG Min(Department of Obstetrics and Gynecology,Chongqing Municipal Hospital of Traditional Chinese Medicine,Chongqing 400021,China;Department of Pathology,Chongqing Municipal Hospital of Traditional Chinese Medicine,Chongqing 400021,China)

机构地区：[1]重庆市中医院妇产科,400021 [2]重庆市中医院病理科,400021

出　　处：《重庆医学》2018年第25期3319-3322,共4页Chongqing medicine

摘　　要：目的探讨葡萄糖转运蛋白3(GLUT3)在胎儿生长受限(FGR)中的表达及意义。方法收集正常妊娠胎盘(n=15)和FGR胎盘(n=15)。采用免疫组织化学法分析GLUT3在胎盘组织母体面和胎儿面表达情况,RT-qPCR及Western blot检测胎盘组织母体面和胎儿面GLUT3 mRNA和蛋白表达情况。结果FGR组胎儿体质量、胎盘质量、胎盘厚度分别为(2 035.9±451.2)g、(386.2±55.3)g、(1.93±0.6)cm均低于对照组(3 205.8±358.1)g、(582.7±77.4)g、(2.56±0.4)cm,差异有统计学意义(P<0.05)。FGR组胎盘组织母体面GLUT3AOD,GLUT3mRNA、蛋白表达水平(0.451±0.121、2.12±0.23、1.12±0.23)明显高于对照组(0.277±0.093、0.46±0.13、0.48±0.13),差异有统计学意义(P<0.05)。FGR组胎盘组织胎儿面GLUT3AOD,GLUT3mRNA和蛋白表达与对照组比较,差异无统计学意义(P>0.05)。结论 FGR胎盘缺氧导致胎盘组织母体面GLUT3表达增高,胎盘因缺氧而过度消耗了葡萄糖,最终可能导致转运到胎儿的葡萄糖降低。Objective To explore the expression of placenta glucose transporter 3(GLUT3)in normal pregnancy and fetal growth restriction(FGR)and to investigate its significance in FGR.Methods The placental tissues were collected from the normal pregnancy group(n=15)and FGR group(n=15).The expression of GLUT3 in placental maternal surface and fetal surface were analyzed by Immunohistochemical method.The expression levels of GLUT3 mRNA and protein in placental maternal surface and fetal surface were detected by adopting RT-qPCR and Western blot respectively.Results The fetal weight,placenta weight and placenta thickness in the FGR group were[(2 035.9±451.2)g,(386.2±55.3)g and(1.93±0.6)cm respectively),which were lower than the corresponding indicators in the control group(3 205.8±358.1)g,(582.7±77.4)g and(2.56±0.4)cm,all P<0.05].The immunohistochemical results showed that the average optical density(AOD)level of GLUT3 in the placental maternal surface of FGR was 0.451±0.121,which was markedly higher than 0.277±0.093 in the control group(P<0.05).The mRNA and protein levels of GLUT3 in placental maternal surface of FGR were 2.12±0.23 and 1.12±0.23 respectively,which were significantly higher than 0.46±0.13 and 0.48±0.13 in the control group(all P<0.05).AOD in the placental fetal surface of FGR,GLUT3 mRNA and protein expression had no significant difference between FGR and control group.Conclusion FGR placental anoxia causes GLUT3 expression increase in placental maternal surface,the placenta excessively consumes glucose due to placental anoxia,and finally leads to decrease of glucose transporting to fetus.

关 键 词：葡萄糖转运蛋白3 胎盘 胎儿生长受限 

分 类 号：R714[医药卫生—妇产科学]