锶胁迫对苗期大豆抗氧化系统的影响  

Effects of Stronium Stress on the Antioxidative System of Soybean(Glycine max)

作　　者：何庆元[1] 向仕华[2] 张超[1] 佟怡馨 孙丽萍 包礼云 吴婷 黄守程[1] HE Qingyuan;XIANG Shihua;ZHANG Chao;TONG Yixin;SUN Liping;BAO Liyun;WU Ting;HUANG Shoucheng(Life Science College,Anhui Science and Technology University,Fengyang 233100,China;Zigong Institute of Agricultural Sciences,Zigong 643000,China)

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100 [2]自贡市农业科学研究所,四川自贡643000

出　　处：《云南农业大学学报（自然科学版）》2018年第4期617-623,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：四川省科技支撑计划(2015NZ0046);安徽科技学院稳定人才项目;安徽科技学院重点学科(AKZDXK2015B02);安徽科技学院大学生创新课题(16XCX50)

摘　　要：【目的】研究稳定性同位素锶对大豆抗氧化系统的影响。【方法】在1/2浓度Hoagland营养液培育的大豆中,用不同浓度(0.00、1.00、5.00、10.00、20.00和40.00 mmol/L)的SrCl_2溶液处理Vc期(单叶半展开,叶片的叶缘已分离),待大豆生长至V2期(单叶以上第一片复叶充分生长),分析不同浓度处理对大豆生长以及POD、SOD和CAT酶活性及同工酶的影响。【结果】SrCl_2处理显著抑制大豆的生长(P<0.05),并随浓度升高抑制作用增强。0.00~10.00 mmol/L范围内SrCl_2处理大豆中POD酶活性迅速升高;此后,随浓度升高,POD酶活性逐渐降低。SrCl_2处理使SOD和CAT酶活性显著升高(P<0.05),并且在5.00 mmol/L达到最大;此后,随浓度升高,酶活性降低。SrCl_2处理大豆的SOD/POD、SOD/CAT和SOD/(POD+CAT)比值都显著降低(P<0.05)。POD同工酶在1.00和5.00 mmol/L SrCl_2处理时出现了1条新的同工酶谱带,但5.00 mmol/L以上浓度处理时POD同工酶谱带减少。SOD和CAT在SrCl_2处理时都出现了1条新的谱带。【结论】大豆能够通过增强抗氧化酶活性来缓解SrCl_2造成的胁迫,然而SOD酶活性增强较小,是造成伤害的限制因素。SrCl_2处理能够影响不同类型的抗氧化同工酶表达。[Purpose]In order to study the effect of strontium on soybean antioxidant system.[Method]The soybean seedlings of Vc period were deal with using different concentration(0.00,1.00,5.00,10.00,20.00 and 40.00 mmol/L)SrCl2 with 1/2-strength Hoagland nutrient solution.The effects on the growth,enzyme activities and isoenzymes of POD,SOD and CAT were analyzed by different treatments until soybean grow to V2 period.[Result]The growth of soybean was significantly inhibited by SrCl2 treatments(P<0.05).The inhibition of soybean growth was greater with increasing SrCl2 concentration.The POD activity rapidly increased from 0.00 to 10.00 mmol/L SrCl2 concentrations and decreased with increasing of SrCl2 concentration when SrCl2 concentration exceeded 10.00 mmol/L.Activities of SOD and CAT of addition SrCl2 treatment were significantly higher than no addition(P<0.05).Two enzyme activities were the highest 5.00 mmol/L SrCl2 concentration.The activities decreased with increasing SrCl2 concentration when SrCl2 concentration exceeded 5.00 mmol/L.The ratio of SOD/POD,SOD/CAT and SOD/(POD+CAT)were significantly diminished in addition SrCl2 treatment(P<0.05).There was one new band of POD isoenzyme in 1.00 and 5.00 mmol/L SrCl2 treatments.The bands of POD isenozyme were reduced more than 5.00 mmol/L SrCl2 concentrations.There was one new band of SOD and CAT isenozyme in SrCl2 treatments,respectively.[Conclusion]The activities of soybean antioxidative enzyme were enhanced to alleviate the stronium stress in SrCl2 treatment.It is limiting factor of alleviation stress that SOD activity enhanced relatively low.SrCl2 treatment could affect the expression of antioxidant isoenzymes.

关 键 词：大豆 锶 抗氧化酶 同工酶 

分 类 号：S565.103.4[农业科学—作物学]