羟丙基-β-环糊精手性流动相添加剂法拆分盐酸氯丙那林  

Enantiomeric Separation of Methocarbamol by HPLC with Chiral Mobile Phase Additives

作　　者：翟明翚[1] 罗梾钰 陈影 王颖[1] 苏立强[1] ZHAI Minghui;LUO Laiyu;CHEN Ying;WANG Ying;SU Liqiang(College of Chemistry and Chemical Engineering,Qiqihar Uiniversity,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《化工技术与开发》2018年第9期1-3,共3页Technology & Development of Chemical Industry

基　　金：黑龙江省教育厅项目(No.135109201;No.135209224)

摘　　要：建立了以羟丙基-β-环糊精(HP-β-CD)为手性流动相添加剂、拆分盐酸氯丙那林的方法。使用C18色谱柱,考察了有机修饰剂的种类及配比、流动相的流速对拆分的影响。结果表明,当流动相为乙腈-甲醇-水(体积比为80∶5∶15),HP-β-CD浓度为0.8mmol·L^(-1),流速为0.8m L·min^(-1),检测波长为216nm时,盐酸氯丙那林的分离度为1.45。The enantiomeric separation of clorprenaline hydrochloride was studied using the hydroxypropyl-β-cyclodextrin(HP-β-CD)as chiral mobile phase additive.The influences of type and concentration of organic modifier,the flow rate on the enantiomers separation were investigated on a C18 column.when The results showed that the mobile phase consist of acetonitrile-methanol-water was 80:5:15(V/V/V),contained 0.8 mmol/L HP-β-CD,at the flow rate of 0.8 mL/min,the detection wavelength was at 216nm,the resolution of clorprenaline hydrochloride enantiomers was 1.45.

关 键 词：羟丙基-Β-环糊精 手性流动相添加剂 拆分 盐酸氯丙那林 

分 类 号：TQ460.72[医药卫生—药物分析学]