KIBRA基因多态性对健康年轻志愿者海马亚区体积影响的研究  

Effects of KIBRA polymorphism on the volume of hippocampus subfield in healthy young volunteers

作　　者：梁雪[1] 王芳芳[1] 王坤[1] 张庆雷[1] 吴思楚 青钊 张鑫[1] 李茗[1] 张冰[1] LIANG Xue;WANG Fang-fang;WANG Kun;ZHANG Qing-lei;WU Si-chu;QING Zhao;ZHANG Xin;LI Ming;ZHANG Bing(Department of Radiology,Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属鼓楼医院,医学影像科,南京210008

出　　处：《磁共振成像》2018年第7期500-505,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金项目(编号:81720108022、91649116、81571040)~~

摘　　要：目的运用磁共振海马亚区体积自动分割方法研究肾脑表达蛋白(kidney and brain expressed protein,KIBRA)rs17070145位点基因多态性对健康年轻志愿者海马亚区体积的影响。材料与方法通过志愿者招募流程,选择符合入组标准的健康年轻志愿者60名纳入本研究。根据志愿者外周血基因型测定结果将受试者分为两组:一组为C等位基因携带者,另一组为TT纯合子携带者。所有受试者均行头颅磁共振高清结构像扫描,其中23名受试者还进行了神经心理学量表评估。利用Free Surfer V5.3.0软件将每一被试的3D-T1加权成像序列颅脑高清结构像进行海马亚区分割及体积计算,分别得到每个被试左右半球海马各亚区体积。将全脑颅内容积(estimated total intracranial volume,eTIV)作为协变量,去除每个被试头部大小不一所导致的海马体积差异,采用线性回归检验对两组受试者进行组间比较,观察海马亚区体积改变模式。再将有统计学差异的海马亚区体积与神经心理学量表行Pearson相关性统计检验,对其相关性进行观察。结果与TT纯合子携带者组相比,C等位基因携带者组左侧CA1区海马亚区体积增大(P=0.032),其他海马亚区体积组间无统计学差异(P>0.05)。并且在23名被试中,左侧CA1区海马亚区体积与数字连线测验-B(NCT-B)完成时间呈明显正相关(r=0.459,P=0.028)。结论在健康年轻受试者中,左侧CA1区海马亚区体积受到KIBRA基因多态性的影响,KIBRA C等位基因携带者左侧CA1区海马亚区体积的增加可能是对空间记忆和执行功能减低的一种代偿作用,该研究结果有助于KIBRA基因多态性与健康年轻人空间记忆和执行功能关系神经机制的研究。Objective:To investigate the effect of kidney and brain expressed protein(KIBRA)rs17070145 gene polymorphism on the volume of hippocampus subfield in healthy young volunteers by using the MR automatic segmentation method.Materials and Methods:A total of 60 healthy young volunteers were recruited in the study according to volunteer recruitment process criterion.Subjects were divided into two groups according to the genotype test:one group was the C allele carriers(CC+CT)and another group was the TT homozygous carriers.All subjects underwent the head magnetic resonance imaging,23 subjects of who were assessed by the neuropsychological scale.The volume of the hippocampal subfields were segmented and calculated by FreeSurfer V5.3.0 software from the 3D-T1 weighted imaging sequence.The differece of each hippocampal subfield volume was compared between the two groups by using the linear regression analysis,controlling for eTIV(estimated total intracranial volume).The eTIV was used as the covariate in this analysis to remove the difference of hippocampal volume caused by the size of each subject.The Pearson correlation test was used between the volume of the hippocampus subfield and the neuropsychological scale.Results:Compared with TT homozygote carriers,the volume of the left CA1 region volume of the C allele group was significantly increased(P=0.032).No significant differences were found between the two groups in other hippocampal subfields.And there was a significant positive correlation between the volume of the left CA1 region volume and the complete time of digital connection test-B(NCT-B)(r=0.459,P=0.028)in 23 volunteers.Conclusions:In the young healthy volunteers,the volume of the left CA1 region is affected by the KIBRA gene polymorphism.The increased volume of left CA1 region in the C allele carriers may be a compensatory effect on the reduction of spatial memory and execution function,which will be helpful to study the neural mechanisms of KIBRA gene polymorphism and spatial memory and execution function on

关 键 词：海马 基因 多态现象 遗传学 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R338.27[医药卫生—诊断学]