体外培养大鼠颌骨成骨细胞成骨及成血管相关因子表达检测  

Expression of Osteogenesis and Angiogenesis Cytokines in Osteoblasts from Rat Mandible

作　　者：陈玉玲[1,2] 许雄程 阳雪 吴玉铭 何梦娇 骆凯[2,3] CHEN Yuling;XU Xiongcheng;YANG Xue;WU Yuming;HE Mengjiao;LUO Kai(Department of Orthodontics,The Affiliated Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China;Key Laboratory of Stomatology of Fujian Provincial University,Fujian Medical University,Fuzhou 350002,China;Department of Periodontologhy,Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China)

机构地区：[1]福建医科大学附属口腔医院正畸科,福州350002 [2]福建医科大学高校口腔医学重点实验室,福州350005 [3]福建医科大学附属口腔医院牙周科,福州350002

出　　处：《福建医科大学学报》2018年第3期139-143,共5页Journal of Fujian Medical University

基　　金：国家自然科学基金(81100761);福建省科技创新联合资金项目(2016Y9023);福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-28)

摘　　要：目的探讨体外培养的大鼠颌骨成骨细胞成骨及成血管相关因子的表达情况。方法体外分离培养大鼠颌骨成骨细胞,采用碱性磷酸酶染色(ALP)与矿化结节茜素红染色对细胞进行鉴定。应用实时荧光定量聚合酶链反应及酶联免疫吸附法检测细胞成骨与成血管相关细胞因子的表达情况。结果体外培养的大鼠颌骨成骨细胞ALP及矿化结节茜素红染色均为阳性。颌骨成骨细胞在高表达成骨相关基因、Runt相关转录因子2、Ⅰ型胶原和骨钙素的同时,在蛋白及基因水平均高表达成血管相关细胞因子血管内皮生长因子、血管生成素1和成纤维生长因子2。结论颌骨成骨细胞在成骨分化的同时可通过分泌成血管相关细胞因子参与血管化调控。Objective To detect the expression of osteogenesis and angiogenesis cytokines in osteoblasts cultured from the rat mandible. Methods Osteoblasts were isolated and cultured from the mandible of rats in vitro. The character of osteoblasts was assessed by ALP staining and mineralized nodules Alizarin red staining. The expressions of osteogenesis and angiogenesis cytokines of osteoblasts were evaluated using Real-time PCR and ELISA. Results ALP staining and mineralized nodules Alizarin red staining were all positive in osteoblasts cultured from the rat mandible. The expression of angiogenesis-related cytokines(VEGF,Angpt-1 and FGF-2)and osteogenesis genes(ALP,RUNX2,Col-Iαand OC)were all up-regulated in osteoblasts. Conclusion Osteoblasts may promote vascularization through angiogenesis-related cytokines secretion.

关 键 词：成骨细胞 成纤维细胞生长因子2 细胞因子类 骨生成 骨形态发生蛋白质类 

分 类 号：R341[医药卫生—基础医学] R329.24