青鹏软膏对组胺诱导急性瘙痒模型小鼠治疗作用  

Antipruritic Effects of Qingpeng Ointment on Acute Itch Model Induced by Histamine in Mice

作　　者：龚璇 刘思思 银量 涂楚月 梅之南[1] 熊慧[1] GONG Xuan;LIU Sisi;YIN Liang;TU Chuyue;MEI Zhinan;XIONG Hui(College of Pharmacy,South-Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《医药导报》2018年第10期1166-1169,共4页Herald of Medicine

基　　金：民族药重大新药创制项目(2017ZX09301060);湖北省技术创新专项(2017AHB067)

摘　　要：目的观察藏药青鹏软膏对由组胺诱导小鼠急性瘙痒的治疗作用并探讨其作用机制。方法 42只雄性C57BL/6小鼠随机分为空白对照组,模型对照组,青鹏软膏小、中、大剂量组(0.625,1.25,2.5 g·kg^(-1)),苯海拉明组(10 mg·kg^(-1)),每组7只。青鹏软膏小、中、大剂量组药物分别涂抹于小鼠后颈部皮肤,连续用药4 d,除了空白对照组,其他所有小鼠右肩部皮内注射组胺500μg(50μL),观察注射组胺后30 min的挠痒次数,检测小鼠皮肤的白细胞介素(IL)-4和胸腺基质淋巴细胞生成素(TSLP)的mRNA表达水平,以及血清中总组胺、IgE、肿瘤坏死因子(TNF)-α、干扰素(IFN)-γ、IL-1β和IL-4浓度。结果与模型对照组比较,青鹏软膏小、中、大剂量组小鼠在组胺注射后30 min内挠痒次数明显降低(P<0.05);小鼠皮肤IL-4 mRNA和TSLP mRNA表达水平明显降低;血清中组胺、IgE以及细胞因子TNF-α、IFN-γ、IL-1β和IL-4的表达水平也显著降低(P<0.05)。结论青鹏软膏对组胺引起的急性瘙痒具有明显治疗作用。Objective To investigate the antipruritic effect of Tibetan medicine Qingpeng ointment in treating histamine-induced acute itch in mice as well as its possible mechanisms.Methods A total of 42 male C57BL/6 mice were randomly divided into blank control group,model control group,low-,medium-and high-dose Qingpeng ointment groups(0.625 g·kg-1,1.25 g·kg-1 and 2.5 g·kg-1),diphenhydramine group(10 mg·kg-1).Mice in Qingpeng ointment group and diphenhydramine group,Qingpeng ointmen and diphenhydramine were applied to pically to the neck of mice for 4 consecutive days,respectively,and then all the mice except blank control group were intradermically injected with 500μg(50μL)histamine.The scratching times in 30 min after histamine injection was recorded.IL-4 and TSLP mRNA levels in the skin and the levels of IgE,histamine,TNF-α,IFN-γ,IL-1βand IL-4 in serum were detected.Results Compared with model control group,scratching times in mice of low-,medium-and high-close Qingpeng ointment group were significantly lower(P<0.05).TSLP mRNA and IL-4 mRNA levels in the skin,and the levels of IgE,Histamine,TNF-α,IFN-γ,IL-1βand IL-4 in the serum were also significantly lower(P<0.05).Conclusion The topical application of Qingpeng ointment has a dramatic therapeutic effect on histamine-induced acute itch in mice.

关 键 词：青鹏软膏 组胺 瘙痒 急性 止痒 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]