川芎嗪对单核细胞系THP-1细胞TLR7/NF-κB表达的影响  

Effect of Ligustrazine on Expression of TLR7/NF-κB in Human THP-1 Cells

作　　者：李续博 姜广宇[2] 赵旭[3] LI Xubo;JIANG Guangyu;ZHAO Xu(Department of TCM Basis,Heilongjiang University of Traditional Chinese Medicine,Haerbin 150040,China;Department of Brain Surgery,the First Affiliated Hospital of Jiamusi University,Jiamusi 154002,China;Department of TCM Clinic,Heilongjiang University of Traditional Chinese Medicine,Haerbin 150040,China)

机构地区：[1]黑龙江中医药大学中医基础部,哈尔滨150040 [2]佳木斯大学附属第一医院脑外科,佳木斯154002 [3]黑龙江中医药大学中医临床部,哈尔滨150040

出　　处：《医药导报》2018年第10期1174-1178,共5页Herald of Medicine

基　　金：黑龙江中医药大学校科研基金资助(201737)

摘　　要：目的探讨川芎嗪对人单核细胞系THP-1细胞TLR7/NF-κB表达的影响。方法将THP-1细胞以每孔2×106个分别接种于6孔细胞培养板。将细胞分为空白对照组,川芎嗪大、中、小剂量组,分别给予不同浓度川芎嗪处理细胞。噻唑蓝(MTT)法观察细胞增殖;TUNEL法检测细胞凋亡;反转录-聚合酶链反应(RT-PCR)法分析TLR7与NF-κB的mRNA表达;Western blotting分析TLR7、NF-κB的蛋白表达。结果与空白对照组比较,川芎嗪低于2.5μg·L^(-1)对细胞活力无明显变化(P>0.05),高于2.5μg·L^(-1)对细胞增殖具有明显抑制作用(P<0.01)。川芎嗪大、中剂量组细胞凋亡率显著升高(P<0.01),川芎嗪大、中剂量组THP-1细胞TLR7与NF-κB mRNA和蛋白表达量显著上升(P<0.01)。川芎嗪小剂量组THP-1细胞TLR7与NF-κB mRNA及蛋白表达量无明显变化(P>0.05)。结论川芎嗪可明显提高细胞凋亡率,其作用机制可能与上调TLR7和NF-κB基因及蛋白表达有关。Objective To investigate effect of ligustrazine on expression of TLR7/NF-κB in human THP-1 cells.Methods The THP-1 cells were inoculated in 6 plates with 2×10 6 per hole.Experiment was divided into blank control group,high-dose,medium-dose and low-dose ligustrazine group.Cell proliferation was determined by MTT method.The cell apoptosis was determined by TUNEL method;mRNA expression of TLR7 and NF-κB was determined by RT-PCR method;the expression of protein of TLR7 and NF-κB was determined by Western blotting.Results Compared with the blank control group,the cell activity was not significantly changed when ligustrazine was less than 2.5μg·L-1(P>0.05),but significantly changed when ligustrazine was higher than 2.5μg·L-1(P<0.01).TLR7 and NF-κB mRNA and protein expression of THP-1 cells in high,middle dose group significanlty increased(P<0.01).TLR7 and NF-κB mRNA and protein expression of THP-1 cells in low dose group was not significantly changed(P>0.05).Conclusion Ligustrazine can significantly improve cell apoptosis rate,and its mechanism may be related to the increase of TLR7 and NF-κB gene and protein expression.

关 键 词：川芎嗪 THP-1细胞 TOLL样受体 白血病 髓系 急性 

分 类 号：R966[医药卫生—药理学]